microRNA-192在结直肠癌中的表达及意义

Expression and clinical significance of microRNA-192 in colorectal carcinomas

目的探讨microRNA-192在结直肠癌组织中的表达及意义。方法选取2010-01—2014-12南方医科大学附属东莞医院手术切除的结直肠癌患者45例,腺瘤25例,增生性息肉25例。采用荧光定量PCR技术检测上述组织中microRNA-192的表达水平。利用脂质体将microRNA-192模拟物瞬时转染至人肠癌细胞株(LoVo)中,采用CCK-8试剂盒和Transwell小室装置,观察其对肠癌细胞增殖和迁移的影响。结果microRNA-192在结直肠癌组织中表达低于腺瘤和增生性息肉(P<0.05)。体外细胞实验显示,上调microRNA-192表达可抑制LoVo细胞增殖和迁移。结论microRNA-192在结直肠癌中呈低表达状态,且与肠癌细胞增殖和迁移相关,有可能成为抗肿瘤治疗的潜在靶点。

Objective To investigate the expression and significance of microRNA-192 in colorectal carcinomas(CRC).Methods The expression of microRNA-192 in 45 primary CRC tissues,25 adenomas and 25 hyperplastic polyps was assessed by real-time fluorecence quantitative PCR.Cell proliferation and migration were measured with the Cell Counting kit-8 and Transwell assay,respectively.Results Lower microRNA-192 expression was found in primary CRC tissues,compared with adenomas and hyperplastic polyps(P<0.05).Transfection of microRNA-192 mimics suppressed proliferation and migration in LoVo cells.Conclusion MicroRNA-192 expression was associated with the progression of colorectal carcinomas,suggesting it to be a potential target point for cancer therapy.