一起中学弯曲菌食源性疾病暴发事件的病原学检测结果

Etiological characteristics of an outbreak of Campylobacter foodborne disease in a middle school

目的分析苏州市某中学一起弯曲菌食源性疾病暴发事件的病原学检测结果,为弯曲菌食源性疾病暴发事件致病因子识别提供参考。方法收集该事件病例肛拭子18份、食堂工作人员肛拭子10份、留样食品43份、饮用水样品2份、食品原材料2份和环境涂抹样品31份,采用胃肠道感染测试条快速筛查病原体,采用双孔滤膜法分离培养病原菌,采用脉冲场凝胶电泳(PFGE)进行聚类分析,采用弯曲菌琼脂稀释法进行药敏试验。结果共报告63例病例,主要临床症状有腹泻,51例占80.95%;发热,39例占61.90%。无住院和死亡病例。分离培养出弯曲菌阳性样本12份,其中11份来源于病例肛拭子,为空肠弯曲菌阳性10份,结肠弯曲菌阳性1份;1份来源于食品原材料,为结肠弯曲菌阳性。PFGE聚类分析结果显示,10株空肠弯曲菌同源性100.0%;对萘啶酸、环丙沙星和四环素耐药率为100.0%,且表现出多重耐药性。结论这是一起由空肠弯曲菌感染引起的食源性疾病暴发事件;采用胃肠道感染测试条、双孔滤膜法分离培养及PFGE分子分型技术能够快速、准确识别致病因子。

ObjectiveTo analyze the etiological characteristics of an outbreak of Campylobacter foodborne disease in a middle school in Suzhou City, so as to provide insights into the identification of pathogenic factors of Campylobacter foodborne disease outbreaks.Methods food samples, 2 drinking water samples, 2 food original material samples and 31 environmental samples were collected,and the pathogens were rapidly screened using the gastrointestinal infection detection strip. The pathogens were isolated and cultured using the double-pore filtration membrane method, and cluster analysis of bacterial isolates was performed using pulsed field gel electrophoresis( PFGE). In addition, the susceptibility of Campylobacter isolates to antibiotics was tested using the Campylobacter agar dilution method.Results reported, and the major clinical symptoms included diarrhea( 51 cases, 80.95%) and fever( 39 cases, 61.90%), while no inpatients or deaths were found. Twelve Campylobacter-positive samples were detected, including 11 anal swabs sampled from patients and one food original material sample. Among the 11 positive anal swabs, there were 10 samples positive for Campylobacter jejuni and one sample positive for C. coli, and of the one positive food original material, C. coli was identified. PFGE analysis showed that 10 C. jejuni isolates of had 100.0% homology, and these 10 isolates were100.0% resistant to naphthyridic acid, ciprofloxacin and tetracycline, appearing multidrug resistance.ConclusionsThis is an outbreak of foodborne disease caused by C. jejuni infections. Gastrointestinal infection detection strips, double-pore filtration membrane and PFGE typing are rapid and accurate to identify pathogenic factors.