高效液相色谱法测定食品中番泻苷A和番泻苷B的含量

Determination of Sennoside A and Sennoside B in Foods by High-Performance Liquid Chromatography

建立高效液相色谱法测定食品中番泻苷A和番泻苷B的方法。样品经过0.2%碳酸氢钠溶液超声提取,采用Agilent ZORBAX SB-C;(4.6 mm×150 mm,5.0 μm)色谱柱分离,以四氢呋喃-水-乙酸为流动相洗脱,DAD检测器,检测波长:340 nm。番泻苷A、番泻苷B在1~100 μg/mL范围线性关系良好,相关系数均大于0.999,检出限(S/N=3)为15 mg/kg,定量限(S/N≥10)为50 mg/kg。以空白样品进行添加回收试验,番泻苷A的回收率为80.88%~96.34%,相对标准偏差(Relative Standard Deviation)为3.07%~7.73%,番泻苷B的回收率为81.32%~102.2%,相对标准偏差(RSD)为2.62%~9.62%。该方法前处理简单、灵敏度高、操作简便、准确性良好。在市售的果冻类食品中,检出阳性样品。

To establish a method for the determination residues of sennoside A and sennoside B in foods by High-performance liquid chromatography (HPLC).The samples were extracted by ultrasonic with 0.2% sodium bicarbonate solution,Agilent Zorbax SB-C;(4.6 mm×150 mm,5.0 μm) chromatographic column was used for the separation,eluted by tetrahydrofuran water acetic acid as mobile phasephase,detected at 340 nm by diode arraydetector (DAD).The calibration curves showed a good linearity in the range of 1~100 μg/mL for sennoside A and sennoside B for perchlorate with r≥0.999.The limit detections of sennoside A and sennoside B (S/N>3) were 15 mg/kg.And the limit quantitatives of sennoside A and sennoside B (S/N>10) were 50 mg/kg.The average recoveries of sennoside A were between 80.88% to 96.34%.The relative standard deviations (RSD) were 3.07%~7.73%.The average recoveries of sennoside B were between 81.32% to 102.2%.The relative standard deviations (RSD) were 2.26%~9.62%.The method is simple,sensitive and accurate.The positive samples were detected in jelly foods on the market.