人脐间充质干细胞外泌体通过miR-126-3p抑制前列腺癌生长的相关研究

Human umbilical cord mesenchymal stem cell exosomes inhibit the growth of prostate cancer by delivering miR-126-3p

目的探究人脐间充质干细胞外泌体(hucMSCs exo)miR-126-3p对前列腺癌生长的影响。方法采用实时荧光定量聚合酶链反应(qRT-PCR)检测RWPE.1､LNCaP､PC-3､C4-2中miR-126-3p表达水平。C4-2细胞中转染miR-126-3p mimic､inhibitor､negative control后检测细胞的增殖水平。提取并鉴定hucMSCs exo,将hucMSCs exo与C4-2细胞共孵育后检测C4-2细胞中miR-126-3p表达水平和细胞的增殖能力,并且将hucMSCs转染miR-126-3p mimic､inhibitor､negative control后提取外泌体,将外泌体与C4-2共孵育后检测miR-126-3p表达水平及细胞的增殖能力。结果miR-126-3p低表达于LNCaP､PC-3､C4-2细胞。miR-126-3p mimic可显著抑制C4-2细胞的增殖能力。与hucMSCs exo共孵育后C4-2 miR-126-3p表达水平显著上调,细胞增殖能力下降,hucMSCs exo可传递miR-126-3p至C4-2细胞,并且外泌体miR-126-3p可显著抑制C4-2细胞的增殖能力。结论hucMSCs exo可通过递送miR-126-3p而抑制前列腺癌细胞的生长。

Objective To explore the effect of human umbilical cord mesenchymal stem cell exosomes(hucMSCs exo)miR-126-3p on the growth of prostate cancer.Methods qRT-PCR was used to detect the expression levels of miR-126-3p in RWPE.1,LNCaP,PC-3,and C4-2.C4-2 cells were transfected with miR-126-3p mimic,inhibitor and negative control,then to detect the proliferation of C4-2 cells.The hucMSCs exo were extracted and identified.After co-incubation of hucMSCs exo with C4-2 cells,the expression level of miR-126-3 p in C4-2 cells and the proliferation ability of the cells were detected.After hucMSCs were transfected into miR-126-3 p mimic,inhibitor,negative control,the exosomes were extracted,and incubated with C4-2,then to detect the expression level of miR-126-3 p and the cell proliferation ability.Results MiR-126-3 p was low expressed in LNCaP,PC-3 and C4-2 cells.miR-126-3 p mimic can significantly inhibit the proliferation of C4-2 cells.After co-incubation with hucMSCs exo,the expression level of C4-2 miR-126-3 p was significantly increased,and cell proliferation decreased.hucMSCs exo can transmit miR-126-3 p to C4-2 cells,and exosomal miR-126-3 p can significantly inhibit the proliferation of C4-2 cells.Conclusions HucMSCs exo can inhibit the growth of prostate cancer cells by delivering miR-126-3 p.