清瘟败毒饮对脓毒症大鼠炎症反应和脏器损伤的影响

Effect of Qingwen Baidu decoction on inflammatory response and organ injuries in rats with sepsis

目的:观察清瘟败毒饮对脓毒症大鼠炎症反应和脏器损伤的影响。方法:将80只大鼠随机分为模型组、西药组及中药高、低剂量组,每组各20只。采用盲肠结扎穿刺术进行造模。西药组灌胃盐酸小檗碱0.27 mg/kg,中药高剂量组灌胃清瘟败毒饮4.6 g生药/kg,中药低剂量组灌胃清瘟败毒饮2.3 g生药/kg,模型组灌胃等量0.9%氯化钠注射液。1次/d,连续3 d。观察比较各组大鼠进食、活动、皮毛色泽等一般情况,血清肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、白细胞介素-17A(IL-17A)水平以及大鼠肺脏、肝脏组织病理情况。结果:模型组出现寒战、呼吸加快、活动度降低、皮毛倒立、精神萎靡等情况,中药高、低剂量组大鼠的一般情况优于模型组;与模型组比较,中药高、低剂量组和西药组均可降低血清TNF-α、IL-1β、IL-6、IL-17A水平(P<0.05),但中药高、低剂量组与西药组比较,指标差异无统计学意义(P>0.05)。中药高、低剂量组肺脏、肝脏组织病理损伤程度较模型组改善。结论:清瘟败毒饮可以抑制脓毒症模型大鼠炎症反应,减轻脏器损伤。

Objective:To investigate the effect of Qingwen Baidu decoction on inflammatory response and organ injuries in rats with sepsis.Methods:A total of 80 rats were randomly divided into model group,Western medicine group,and high-and low-dose traditional Chinese medicine(TCM)groups,with 20 rats in each group,and cecal ligation and puncture were performed for modeling.The rats in the Western medicine group were given berberine hydrochloride 0.27 mg/kg by gavage,those in the high-and low-dose TCM groups were given Qingwen Baidu decoction by gavage at a dose of 4.6 and 2.3 g/kg(crude drug),respectively,and those in the model group were given an equal volume of 0.9% sodium chloride injection by gavage,once a day for 3 consecutive days.The above groups were compared in terms of general status(food intake,activity,and fur color),serum levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-10,and interleukin-17 A(IL-17 A),and lung/liver histopathology.Results:The rats in the model group had the conditions of chill,accelerated breathing,reduced activity,hair upside down,and lethargy,and the high-and low-dose TCM groups had a better general status than the model group;compared with the model group,the high-and low-dose TCM groups and the Western medicine group had significant reductions in the serum levels of TNF-α,IL-1β,IL-6,and IL-17 A(P<0.05),while there were no significant differences in these indices between the high-and low-dose TCM groups and the Western medicine group(P>0.05).The high-and low-dose TCM groups had significant improvement in lung/liver histopathology compared with the model group.Conclusion:Qingwen Baidu decoction can inhibit inflammatory response and alleviate organ injuries in rats with sepsis.