玉米天冬氨酸蛋白酶基因家族的鉴定与表达分析

Genome-Wide Identification and Expression Analysis of Aspartic Protease Family in Maize

以玉米B73基因组为参考序列,在全基因组水平系统分析玉米天冬氨酸蛋白酶家族成员的基因组结构信息,深入了解玉米天冬氨酸蛋白酶家族基因(ZmAPs)的生物学功能。结果表明,总共鉴定出75个ZmAPs,通过系统进化树分析将ZmAPs分为两大亚组,同一亚组之间的motif分布与基因结构都相对保守。染色体定位和共线性分析发现,ZmAPs不均匀地分布在玉米的10条染色体上,其中,7对为串联重复基因。对其启动子顺式作用元件分析后发现,ZmAPs可能参与光响应、MeJA及ABA等信号通路。对生物与非生物胁迫下的玉米转录组表达谱分析发现,ZmAPs对生物胁迫的响应更为强烈,表明ZmAPs可能参与玉米抗病的过程。

To explore the biological functions of ZmAPs,the B73 maize genome was used as a reference genome to identify the maize Aspartic protease family members by genome-wide gene structure analysis.In this study,a total of 75 ZmAPs were identified,and ZmAPs were divided into two major subgroups by phylogenetic tree analyses.Motif distribution and gene structure in the same subgroup was relatively conservative.Chromosomal mapping analysis showed that ZmAPs were unevenly distributed in 10 different maize chromosomes and 7 pairs of tandem repeats were found.After analyzing the cis-elements of the gene promoters,we found that ZmAPs may be involved in light response,MeJA and ABA signaling pathway etc.In addition,through transcriptome profiling of maize under biotic and abiotic stress,we found that ZmAPs were more responsive to biological stress,suggesting that ZmAPs may be involved in disease resistance in maize.