吉林省内花生衣药材中黄曲霉毒素B1、B2、G1、G2的测定

Determination of Aflatoxin B1,B2,G1 and G2 in Testa Arachis by HPLC-Post-Colum Photochemical Derivatization

为测定吉林省内花生衣药材中黄曲霉毒素B1、B2、G1、G2的含量,评估样品污染情况,本文采用免疫亲和柱净化、高效液相色谱光化学衍生荧光检测法,以Agilent ZORBAX SB-C18为色谱柱(4.6×250 mm 5μm);以甲醇-乙腈-水(40∶20∶40)为流动相,流速为0.8 mL/min;柱温为25℃;荧光检测器激发波长λex=360 nm,发射波长λex=450 nm。结果显示,AFB1、AFB2、AFG1、AFG2线性关系良好;AFB1、AFB2、AFG1、AFG2检出限依次为0.155μg/kg、0.050μg/kg、0.155μg/kg、0.058μg/kg;定量限依次为0.465μg/kg、0.150μg/kg、0.465μg/kg、0.175μg/kg。由此可见,该方法快速、准确、重复性好,能够为花生衣中黄曲霉毒素测定方法提供科学依据。

In order to determine the contents of aflatoxin B1,B2,G1 and G2 in peanut coating medicinal materials in Jilin Province and evaluate the pollution of samples,immunoaffinity column purification and high performance liquid chromatography photochemical derivatization fluorescence detection were used,the colum was Agilent ZORBAX SB-C18 chromatographic column(4.6×250mm,5μm);The mobile phase consisted of methanol-acetomitrile-water(40:20:40);the flow velocity was 0.8ml,Column temperature was 25℃.Excitiation wavelength and emission wavelength of fluorescence detector were 360 nm and 450 nm.The results show that AFB1,AFB2,AFG1,AFG2 showed good linearity.The detection limit of AFB1,AFB2,AFG1 and AFG2 were 0.155μg/kg,0.05μg/kg,0.155μg/kg,0.058μg/kg.The quantitative limits were 0.465μg/kg,0.150μg/kg,0.465μg/kg and 0.175μg/kg,.Thus it can be seen,the method is fast,accurate and reproducible,which provides a scientific basis for the determination of aflatoxin in testa arachis.