脂氧素A4通过影响炎症因子表达对心肌细胞缺氧/复氧损伤的保护作用

Lipoxin A4 protects cardiomyocytes from hypoxia/reoxygenation injury by affecting the expression of inflammatory factors

目的探讨脂氧素A4(LXA4)在大鼠心肌细胞缺氧/复氧损伤中的保护作用及可能炎症因子调解机制。方法将大鼠心肌细胞H9C2随机分为三组,每组6孔,分别为空白对照组(Control组)、缺氧/复氧组(H/R组)、LXA4处理组(LXA4+H/R组),SP法观察每组心肌细胞中NF-κB、TNF-α、IL-1β、IL-10的表达情况,同时RT-PCR法检测NF-κB mRNA的表达情况,提取上清液ELISA测定TNF-α、IL-1β、IL-10的浓度。结果与H/R组相比Control组和LXA4+H/R组心肌细胞中IL-10的表达明显增强,NF-κB、TNF-α、IL-1β表达明显减弱,同时H/R组NF-κB mRNA表达水平升高,上清液中的TNF-α、IL-1β、IL-10浓度与心肌细胞内的表达趋势相同。结论LXA4可抑制缺氧/复氧心肌细胞中炎症因子的表达,同时上调抑炎因子,起到减轻心肌细胞缺氧/复氧损伤作用。

Objective To investigate the protective effect of 1ipoxin A(LXA4)on hypoxia/reoxygenation injury of rat cardiomyocytes and the possible mediating mechanism of inflammatory factors.Methods The rat myocardial cell H9C2 divide into 3 groups at random,blank Control group(Control group),hypoxia/reoxygenation(H/R group),LXA4 treatment group(LXA4+H/R group),each group of 6 holes,SP method to observe the myocardial cells in each group the expression of NF-κB、TNF-α、IL-1β、IL-10,at the same time RT-PCR method to detect the NF-κB mRNA expression,extract that ELISA determination the concentrationof TNF-α、IL-1β、IL-10 in supernatant fluid.Results Compared with the Control group and the LXA4+H/R group,the expression ofIL-10 in cardiomyocytes in the Control group and the LXA4+H/R group was significantly enhanced,the expression of NF-κB、TNF-α、IL-1βwas significantly decreased,and the expression level of NF-κB mRNA was increased.The concentration of TNF-α、IL-1β、IL-10 in the supernatant was the same as that in cardiomyocytes.Conclusion LXA4 can inhibit the expression of inflammatory factors in anoxia/reoxygenation cardiomyocytes,and up-regulate the inhibitory inflammatory factors to play a role in reducinganoxia/reoxygenation injury of cardiomyocytes.