黄芪丹参对妊娠高血压大鼠胎盘VEGF、TNF-α表达的影响

Effect of astragalus and salvia miltiorrhiza on VEGF and TNF levels in placenta of rats with pregnancy induced hypertension

目的研究黄芪丹参对妊娠高血压大鼠胎盘血管内皮生长因子(Vascular endothelial growth factor, VEGF)、肿瘤坏死因子α(Tumor necrosis factorα,TNF-α)水平的影响。方法建立妊娠高血压大鼠模型,30只Wistar大鼠随机分为对照组(NC组,n=10)、妊娠高血压大鼠模型组(HDCP组,n=10)和妊娠高血压大鼠模型组加黄芪丹参治疗组(HQ组,n=10)。采用无创血压仪检测大鼠血压;BCA法测定24 h尿蛋白含量;HE染色观察胎盘组织的病理改变;ELISA法检测血清中VEGF含量;放射免疫分析法测定血清中TNF-α含量;Western blot检测大鼠胎盘组织中VEGF和TNF-α蛋白的表达。结果在妊娠第16、17、21天时,与NC组相比,HDCP组大鼠的血压情况明显上升(P<0.05);与HDCP组相比,HQ组大鼠的血压显著降低(P<0.05)。在妊娠第16、17、21天时,HDCP组大鼠的尿蛋白含量显著比NC组增多(P<0.05);经过黄芪丹参治疗后HQ组大鼠的尿蛋白含量明显降低(P<0.05)。与NC组相比,HDCP组胎盘绒毛数目减少结构萎缩,部分绒毛纤维素样坏死,滋养层细胞代偿性增值。HQ组较HDCP组绒毛纤维素样程度减轻,情况明显好转。与NC组相比,HDCP组大鼠血清中VEGF含量显著降低,TNF-α含量显著增多(P<0.05);干预后的HQ组VEGF含量显著多于HDCP组,TNF-α含量显著低于HDCP组(P<0.05)。HDCP组大鼠胎盘组织中VEGF表达低于NC组(P<0.05),TNF-α蛋白明显高于NC组(P<0.05);药物干预后,HQ组VEGF表达显著增加(P<0.05),同时TNF-α蛋白明显得到抑制(P<0.05)。结论黄芪丹参注射液能促进胎盘组织中VEGF的表达,同时抑制TNF-α的表达,从而对妊娠高血压起到一定的治疗作用。

Objective To study the effect of Astragalus salvia injection on the levels of vascular endothelial growth factor(VEGF) and tumor necrosis factor-α(TNF-α) in placenta of pregnancy-induced hypertension rats.Methods Wistar rats were divided into control(NC,n=10),pregnancy-induced hypertension rat model(HDCP,n=10) and HDCP received Astragalus salvia injection treatment(HQ,n=10) groups.The blood pressure was measured by a non-invasive blood pressure instrument;the 24-h urine protein content was measured by the BCA method;the pathology of the placental tissue were observed by HE staining;the serum VEGF and TNF-α content was measured by the ELISA and radioimmunoassay, respectively;The expression of VEGF and TNF-α protein in placenta was determined by Western blot.Results At 16,17 and 21 days of pregnancy, compared with the NC group, the blood pressure of rats in the HDCP group was increased(P<0.05);which was decreased in the HQ group.Urinary protein in the HDCP group was higher than that in the NC group(P<0.05),which was decreased in the HQ group.Compared with the NC group, the HDCP group had fewer placental villi, structural atrophy, some cellulose-like necrosis of villi, and compensatory increase in trophoblast cells.These pathological lesions were ameliorated in the HQ group.Compared with the NC group, serum VEGF content of the HDCP group was reduced, while TNF-α content increased(P<0.05);which were attenuated in the HQ group as compared to the HDCP Group(P<0.05) The expression of VEGF in the placenta of HDCP group was lower while TNF-α was higher than that of NC group(P<0.05);which were reversed in HQ group.Conclusion Astragalus salvia injection was effective in the treatment of pregnancy-induced hypertension in rats, and the mechanism appeared to be due to increased expression of VEGF and decreased expression of TNF-α in the placenta.