多肽K-YFAE对H9C2心肌细胞增殖及缺氧耐受的影响

Effects of Polypeptide K-YFAE on Proliferation and Hypoxia Tolerance of H9C2 Cardiomyocytes

为了观察多肽K-YFAE对H9C2心肌细胞增殖和缺氧耐受的影响,体外培养H9C2心肌细胞后,将其分为对照组和多肽组,对照组只使用10%的二甲基亚砜(dimethyl sulfoxide, DMSO)和胎牛血清(fetal bovine serum,FBS)处理,多肽组在对照组处理的基础上分别加入浓度为10μmol/L、20μmol/L、50μmol/L的多肽K-YFAE,7 d后对各组细胞分别行CCK-8检测、细胞周期的流式细胞术检测、细胞周期相关基因的qPCR检测等,以观察多肽K-YFAE对H9C2心肌细胞增殖的影响;用三气培养箱对细胞行缺氧处理后分组,并分别行细胞凋亡的TUNEL检测和流式细胞术检测,以及细胞凋亡相关基因的qPCR检测等,以观察多肽K-YFAE对H9C2心肌细胞缺氧耐受的影响。结果显示:与对照组比较, K-YFAE的干预可促进H9C2心肌细胞进入增殖期,并且减少其凋亡。实验结果初步表明,多肽K-YFAE既可以促进H9C2心肌细胞增殖,又可以增加其缺氧耐受。

To observe the effects of polypeptide K-YFAE on the proliferation and hypoxia tolerance of H9C2 cardiomyocytes, H9C2 cardiomyocytes were cultured in vitro and divided into control and polypeptide groups.In the control group, only 10% dimethyl sulfoxide(DMSO) and fetal bovine serum(FBS) were used, and in the polypeptide groups, different concentrations(10 μmol/L, 20 μmol/L and 50 μmol/L) of polypeptide K-YFAE were added. After 7 days, CCK-8, flow cytometry and qPCR were conducted to observe the effects of K-YFAE on the proliferation of H9 C2 cardiomyocytes. After hypoxia treatment in three-gas incubator, the cells were grouped, for apoptosis detection by both TUNEL assay and flow cytometry, and for apoptosis-related genes by qPCR. The results showed that, compared with the control group, the intervention of K-YFAE promoted H9 C2 cardiomyocytes to enter the proliferation phase and decreased their apoptosis, which means that K-YFAE may not only promote the proliferation of H9 C2 cardiomyocytes, but also increase their hypoxia tolerance.