口腔癌临床病理标本中PD-L1表达水平

Expression level of PD-L1 in clinicopathological specimens of oral cancer

目的探讨口咽癌、上颌窦癌、舌癌等口腔癌的病理组织中程序性死亡蛋白配体-1(PD-1)蛋白的表达情况。方法2015年1月至2020年1月,自北京怀柔医院收集20例确诊为口腔癌的病理组织,通过免疫组织化学法对其石蜡切片进行PD-L1染色,所有切片于光学显微镜下随机选取10个组织区域进行摄片,并分别计算阳性细胞比例后取平均值,依照评价标准对PD-L1的阳性等级进行定性分析。结果在本研究所收集的20例口腔癌病理组织中,PD-L1强阳性表达共计6例(30%),PD-L1中等阳性表达共计4例(20%),PD-L1弱阳性表达共计4例(20%),PD-L1阴性表达共计6例(30%),PD-L1阳性表达14例(70%)。结论口咽癌、上颌窦癌、舌癌等口腔癌的发病机制也有可能是肿瘤细胞高表达PD-L1引起免疫逃逸造成的。

Objective To investigate the expression of programmed death ligand-1(PD-1)protein in the pathological tissues of oral cancer,such as oropharyngeal cancer,maxillary sinus cancer and tongue cancer,etc.Methods From January 2015 to January 2020,20 pathological tissues diagnosed with oral cancer were collected from Beijing Huairou Hospital,and their paraffin sections were stained with PD-L1 by immunohistochemistry method.All sections were photographed in 10 tissue regions randomly selected under an optical microscope,and the proportion of positive cells was calculated and the average value was taken.The positive grade of PD-L1 was qualitatively analyzed according to the evaluation criteria.Results Among the 20 cases of oral cancer pathological tissues collected in this study,six cases(30%)showed strong positive expression of PD-L1,four cases(20%)showed moderate positive expression of PD-L1,four cases(20%)showed weak positive expression of PD-L1,and six cases(30%)showed negative expression of PD-L1.A total of 14 cases(70%)showed positive expression of PD-L1.Conclusion The pathogenesis of oropharyngeal cancer,maxillary sinus cancer,tongue cancer and other oral cancers may also be caused by immune escape caused by high expression of PD-L1 in tumor cells.