喉癌组织中miR-340-5p、c-Myc、TCF-4的表达及临床意义

Expression and clinical significance of miR-340-5p,c-Myc,and TCF-4 in laryngeal cancer tissues

目的研究喉癌组织中微RNA(miR)-340-5p、原癌基因c-Myc及转录因子4(TCF-4)的表达,分析三者表达的相关性及与喉癌患者临床病理特征的关系。方法选取2015年2月至2020年11月在盘锦辽油宝石花医院诊治的102例喉癌患者为研究对象。采用实时荧光定量逆转录聚合酶链反应法检测102例喉癌患者癌组织及癌旁组织中miR-340-5p、c-Myc、TCF-4的表达,统计分析三者表达与临床病理特征关系,Pearson线性相关分析三者表达的相关性。结果与癌旁组织比较,癌组织中miR-340-5p的相对表达量均明显较低,而c-Myc、TCF-4的相对表达量明显较高(P<0.05)。不同肿瘤分期的喉癌组织中miR-340-5p、c-Myc及TCF-4的表达比较,差异有统计学意义(P<0.05),不同性别、年龄、分型、肿瘤分化程度及是否伴有淋巴结转移比较,差异无统计学意义(P<0.05)。miR-340-5p和c-Myc、TCF-4的表达呈负相关(r=-0.549、-0.452,P<0.05)。结论喉癌癌组织中miR-340-5p表达降低,而c-Myc、TCF-4表达上调,miR-340-5p、c-Myc及TCF-4均与喉癌肿瘤分期有关,参与喉癌的发生发展。

Objective To study the expression of microRNA(miR)-340-5p,proto-oncogene c-Myc and transcription factor-4(TCF-4)in laryngeal cancer tissues,and to analyze the correlation between the expression of them and the clinicopathological characteristics of laryngeal cancer.Methods A total of 102 patients with laryngeal cancer treated in Panjin Liaoyou Baoshihua Hospital from February 2015 to November 2020 were selected as the research subjects.The expressions of miR-340-5p,c-Myc,TCF-4 in cancer tissues and para-cancer tissue of 102 patients with laryngeal cancer were detected by real time fluorescence quantitative reverse transcription polymerase chain reaction.The correlation between the expressions of them and clinicopathological characteristics was statistically analyzed,and the correlation between the expressions of them was analyzed by Pearson linear correlation.Results Compared with para-cancer tissues,the relative expression levels of miR-340-5p in cancer tissues were significantly lower,while the relative expression levels of c-Myc and TCF-4 were significantly higher(P<0.05).There were statistically significant differences in the expressions of miR-340-5p,c-Myc,and TCF-4 in laryngeal cancer tissues with different tumor stages(P<0.05),while there were no statistically significant differences in gender,age,parting,tumor differentiation degree and whether or not lymph node metastasis(P<0.05).The expression of miR-340-5p was negatively correlated with c-Myc and TCF-4(r=-0.549,-0.452,P<0.05).Conclusion The expression of miR-340-5p is decreased in laryngeal cancer tissues,while the expression of c-Myc and TCF-4 is up-regulated.miR-340-5p,c-Myc,and TCF-4 are all related to the tumor stages of laryngeal cancer and are involved in the occurrence and development of laryngeal cancer.