莲子心水提取物正丁醇部位化学成分研究

Chemical constituents from the N-butanol faction of Nelumbinis Plumula aqueous extracts

目的:研究睡莲科莲属植物莲成熟种子中莲子心中水提取物正丁醇部位的化学成分。方法:采用硅胶、反相C;硅胶、Sephadex LH-20凝胶、制备型HPLC等色谱分离技术对莲子心水提物正丁醇部位化学成分进行了分离纯化,并通过波谱学方法(MS、NMR)进行结构鉴定。结果:从莲子心水提物正丁醇部位分离鉴定了18个化合物,分别为Plumumbinis A(1)、甲基莲心碱(2)、莲心碱(3)、异莲心碱(4)、甲基紫堇杷灵碱(5)、3,4-二氢-6,7-二甲氧基-2-甲基-异喹啉(6)、N-甲基异乌药碱(7)、去甲衡州乌药碱(8)、N-甲基紫堇定(9)、白蓬叶灵(10)、吡啶-4-O-葡萄糖苷(11)、β-胡萝卜苷(12)、淫羊藿次甙D2(13)、对羟基肉桂醛(14)、对羟基苯丙酸(15)、异槲皮苷(16)、对香豆酸-4-O-β-D-吡喃葡萄糖苷(17)及棕榈酸(18)。结论:Plumumbinis A(1)为新化合物,化合物6、10、11、13、17、18为首次从莲子心中分离得到。

Objective:To investigate chemical constituents of N-butanol parts of Nelumbinis Plumula aqueous extracts.Methods:Chemical constituents were investigated by various column chromatography on silica gel,ODS,Sephadex LH-20 and preparative HPLC,and their chemical structures were identified by spectral analyses(MS,NMR).Results:Eighteen compounds were isolated and elucidated,including Plumumbinis A(1),Neferine(2),Liensinine(3),Isoliensinine(4),Methylcorypalline(5),3,4-dihydro-6,7-dimethoxy-2-methyl-isoquinolinium(6),N-methylisococlaurine(7),Higenamine(8),N-methylcorydaldine(9),Thalifoline(10),pyridine-4-O-glucoside(11),methylarbutin(12),icariside D2(13),hydroxycinnamaldehyde(14),hydrocinnamic acid(15)isoquercitrin(16),coumaric acidβ-glucopyranoside(17),hexadecanoic acid(18).Conclusion:Plumumbinis A(1)is a new compound,Compounds 6,10,11,13,17,18 are obtained from the plants of Nelumbinis Plumula for the first time.