中华猕猴桃果仁非饱和脂肪酸(果王素)在大鼠放射性肺损伤中的防治作用及其机制探索

Preventive and therapeutic effects of the Kiwi fruit essence(unsaturated fatty acid of actinidia chinesis planch seed oil)on radiation-induced lung injury rats

目的:观察中华猕猴桃果仁非饱和脂肪酸(果王素)在大鼠放射性肺损伤中的防治作用,并通过检测肺组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、髓过氧化物酶(MPO)和转化生长因子-β1(TGF-β1)探讨其机制。方法:将90只Sprague-Dawley大鼠按随机数字表法分为空白组、模型组、果王素组(60、120、240 mg/kg),每组18只。除空白组外,其余组大鼠予以6MV-X线全胸部放射(3Gy/次,2次/周,总剂量18Gy)构建放射性肺损伤大鼠模型,各果王素组在放射前一周分别按60、120、240 mg/kg果王素剂量加入0.9%氯化钠1 mL每日灌胃1次,空白组、模型组在同一时间予以0.9%氯化钠1 mL灌胃1次。各组分别于放射后14 d、28 d、56 d随机处死动物6只,取肺组织行HE染色、Masson染色观察病理学改变,并检测肺组织SOD、GSH-Px、MPO含量及TGF-β1的表达。结果:与模型组相比,60、120、240 mg/kg果王素可显著减轻肺泡炎和放射性肺纤维化程度,降低肺组织羟脯氨酸(HYP)含量,增加抗氧化酶SOD、GSH-Px含量及减少氧化酶MPO含量,下调TGF-β1的表达,且剂量越高效果越明显。结论:果王素对放射性肺损伤具有防治作用,其机制可能与减少氧化应激损伤、下调TGF-β1表达有关。

AIM:To observe the effects of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),myeloperoxidase(MPO)and transforming growth factor-β1(TGF-β1)of radiation-induced lung injury rats by Kiwi fruit essence(unsaturated fatty acid of actinidia chinesis planch seed oil).METHODS:According to random number table,90 Sprague-Dawley rats were divided into the control group,model group,60 mg/kg unsaturated fatty acid of actinidia chinesis planch seed oil intervention group,120 mg/kg unsaturated fatty acid of actinidia chinesis planch seed oil intervention group,240 mg/kg unsaturated fatty acid of actinidia chinesis planch seed oil intervention group,18 animals were included in each group.Except for the control group,rats in the remaining groups were constructed by 6MV-X-ray 18Gy full chest radiation,one week before modeling,the rats in the last 3 groups were given(60,120,240)mg/kg unsaturated fatty acid of actinidia chinesis planch seed oil.The first two groups were given 0.9%sodium chloride solution by gavage,once a day in each rat.14 days,28 days,and 56 days after radiation,the rats were randomly sacrificed and their chests were cut,and ave lung tissue was saved.HE and Masson staining were performed to observe the pathological changes,and the content of SOD,GSH-Px,MPO was determined.The expression of TGF-β1 was analyzed by immunohistochemical staining.RESULTS:Compared with the model group,(60,120,240)mg/kg unsaturated fatty acid of actinidia chinesis planch seed oil significantly reduced the degree of lung alveolitis and radiation pulmonary fibrosis,reduced the content of hydroxyproline(HYP),MPO,increased the antioxidant enzymes SOD and GSH-Px content,down-regulated the expression of TGF-β1.There were significant differences in the above-mentioned indicators among the intervention groups of(60,120,240)mg/kg unsaturated fatty acid of actinidia chinesis planch seed oil group,and it was positively correlated with dosage.CONCLUSION:Unsaturated fatty acid of actinidia chinesis planch seed oil has a preventive effect on radiation-induced lung injury,and its mechanism may be related to the reduction of oxidative stress damage and down-regulation of TGF-β1 expression.