细圆藤醇提物急性毒性研究及抗炎镇痛有效部位筛选

Study on acute toxicity of ethanol extract of Pericampylus glaucus and screening of effective anti-inflammatory and analgesic components

对细圆藤(Pericampylus glaucus)醇提取物的急性毒性进行初步研究,筛选出细圆藤抗炎、镇痛有效部位,为新药的开发提供理论依据。采用最大给药量测定法,小鼠灌胃给药测定藤水提取物和醇提物的半数致死量(LD_(50))或最大耐受量(MTD)研究其安全性;采用冰醋酸致小鼠疼痛和小鼠热刺激反应考察细圆藤不同提取部位的镇痛作用;采用琼脂肉芽肿胀法观察细圆藤不同提取部位的抗炎作用。结果显示,在急性毒性中,未测得半数致死量LD_(50),细圆藤醇提取物的MTD>94 g/kg,最大耐受量倍数相当成人(60 kg)用量的376倍;细圆藤不同部位组均能减轻小鼠肉芽湿重,细圆藤石油醚部位、乙酸乙酯部位、正丁醇部位、水部位的抑制率分别为13.74%、21.35%、16.35%、32.27%,其差异均具有统计学意义;与空白对照组比较,细圆藤石油醚部位、水部位在给药后90、120 min痛阈值显著提高;细圆藤乙酸乙酯部位、正丁醇部位在给药后120 min痛阈值显著提高,细圆藤不同部位组均能减轻醋酸引起小鼠疼痛,其中细圆藤石油醚部位、乙酸乙酯部位、正丁醇部位、水部位的镇痛率分别为51.74%、37.63%、32.13%、40.55%,与空白组比较其差异具有统计学意义;细圆藤无明显毒性,临床常用量安全可行;在抗炎镇痛有效部位筛选发现,细圆藤不同部位均具有一定的抗炎镇痛作用,细圆藤石油醚部位镇痛效果最强,水部位抗炎效果最强。

To study the acute toxicity of alcohol extract of Pericampylus glaucus,the effective anti-inflammatory and analgesic parts of Pericampylus glaucus were screened out to provide theoretical basis for the development of new drugs.The maximum dose assay was used to determine the safety of the water extract and alcohol extract by intragastric administration.The analgesic effects of different extract parts of Pericampylus glaucus were investigated by glacial acetic acid-induced pain in mice and thermal stimulation in mice.The anti-inflammatory effects of different extract parts of Pericampylus glaucus were observed by AGAR granulomatous method.The results showed that in acute toxicity,the lethal dose(LD_(50))was not measured,the MTD of the alcohol extract was more than 94 g/kg,and the maximum tolerance was 376 times of the adult dosage(60 kg).The inhibition rates of petroleum ether,ethyl acetate,n-butanol and water were 13.74%,21.35%,16.35%and 32.27%,respectively,with statistical significance.Compared with blank control group,the pain threshold of petroleum ether and water parts was significantly increased 90 and 120 min after administration.The pain threshold of ethyl acetate part and n-butanol part of Pericampylus glaucus significantly increased 120 min after administration,and different parts of Pericampylus glaucus could relieve the pain caused by acetic acid in mice.The analgesic rates of petroleum ether part,ethyl acetate part,N-butanol part and water part were 51.74%,37.63%,32.13%and 40.55%,respectively.Compared with blank group,the difference was statistically significant.There was no obvious toxicity,and the usual clinical dosage was safe and feasible.In the screening of effective anti-inflammatory and analgesic parts,it was found that the different parts of Pericampylus glaucus had certain anti-inflammatory and analgesic effects,the oil ether part of Pericampylus glaucus had the strongest analgesic effect,and the water part had the strongest anti-inflammatory effect.