酶解预处理工艺在烟叶茄尼醇提取中的应用

Application of Enzyme Pretreatment Process in Extracting Solanesol from Tobacco Leaves

为提高烟叶中茄尼醇的提取率,通过纤维素酶-半纤维素酶联用的方法对烟叶进行预处理,研究了酶种类、添加量、温度、pH和反应时间对烟叶提取液中游离茄尼醇和总茄尼醇含量的影响,并以Box-Behnken响应面法优化酶解工艺。结果表明,在单因素试验中,最优工艺为纤维素酶-半纤维素酶联用(酶添加量为每克烟叶各1.00 U)、温度55℃、pH 5.2和反应时间60 min;与未经酶解预处理的空白组(CK)相比,纤维素酶-半纤维素酶联用处理后,游离和总茄尼醇提取率分别增加305.61%和113.55%,响应面法优化后,总茄尼醇提取率较CK提高158.78%。本研究探索出一种提高茄尼醇提取率的有效方法,对烟草综合利用具有指导意义。

In this study,both cellulase and hemicellulose were used in pretreatment process in order to improve the extraction rate of solanesol in tobacco leaves.The effects of enzyme types,dosage,temperature,pH and reaction time on the content of free solanesol and total solanesol in tobacco leaf extracts were studied.Optimization of enzymatic hydrolysis was carried out by the Box-Behnken response surface method.The results of single-factor experiment showed that the optimal enzymatic hydrolysis process was cellulasehemicellulase combined use(1.00 U per gram of tobacco leaves),temperature 55 ℃,pH 5.2 and reaction time 60 min;Compared with the control group without enzymatic hydrolysis pretreatment,the extraction rate of free solanesol of the cellulase-hemicellulase combined use group was increased by 305.61% and the total solanesol was increased by 113.55%.After response surface optimization,the total solanesol extraction rate was increased by 158.78% compared with the control group.This study has explored an effective way to improve the extraction rate of solanesol in tobacco leaves,which has guiding significance for the comprehensive utilization of tobacco.