新型可降解编织结构神经再生导管的制备及其性能

Fabrication of novel biodegradable braided nerve grafts for nerve regeneration

为研制具有良好力学性能和生物相容性的人工神经导管,采用编织工艺、静电纺丝技术和冷冻干燥技术制备一种含有壳聚糖涂层-编织层-纤维海绵层的3层复合结构人工神经导管,研究轴纱、内外层结构和镁离子质量浓度对其性能的影响,并对导管的表面形貌、力学性能、离子缓释性能和生物相容性进行表征。结果表明:轴纱和编织纱共同参与编织时,神经导管形变50%的径向压缩性能为1.3 N,轴纱断裂时的轴向拉伸应力为30 N,具有良好的力学性能;导管内海绵层呈相互连通的多孔结构,孔径分布均匀(0.04~0.08 mm);导管内镁离子可缓慢释放28 d;当镁离子溶液质量浓度为0.02 g/mL时,导管对细胞增殖的促进作用最显著。这为神经导管的材料选择和结构优化提供新思路。

In order to develop artificial nerve guidance conduits(NGCs)with good mechanical properties and biocompatibility,this paper describes a three-layer composite artificial nerve graft made up with a chitosan coating layer,a braided layer and a fibrous sponge layer using traditional braiding,electrospinning and freeze-drying techniques.The morphology,mechanical properties,biocompatibility and sustained-release properties were investigated,studying the effects of axial yarn,the outer and inner layers and magnesium ion concentration on its performance.The results showed that the prepared NGCs with braided and axial yarns possess good mechanical properties.The radial compressive property of the NGC with 50%deformation is 1.3 N and the axial tensile stress to yarn fracture is 30 N.The sponge layer features an inter-connected porous structure with uniform pore size distribution(0.04-0.08 mm).The magnesium ion in the NGC can be sustainably released for 28 d.When the concentration of magnesium ion solution is 0.02 g/mL,the NGC has the greatest effect on promoting cell proliferation.This paper provides new ideas for selecting materials for NGCs and optimizing their structural properties.