人体蠕形螨标本制作方法探讨

A specimen preparation method for human Demodex

目的探索和研究安全、快速,且能长期保存人体蠕行螨标本的制作方法,以期满足人体寄生虫实验教学的需要。方法采用透明胶纸粘贴法和挤刮涂片法获取蠕形螨,使用多种方法对人体蠕形螨进行处理,对比中性树胶、无味型封片剂以及甘油明胶的封片效果,在数码显微镜KoPa WiFi EDU软件系统下观察和拍摄。结果背景杂质少油脂少不影响观察的标本可直接采用无味型封片剂或者甘油明胶对透明胶封片保存。透明胶未粘贴好导致气泡多或者油脂、汗毛多影响观察的标本可采用2%洗洁精短时间清洁后,依次放入浓度为75%~100%的乙醇溶液中脱水后,采用无味型封片剂封片保存。对于透明胶上密度较大的标本,可采用2%洗洁精长时间浸泡后,离心沉淀,取沉渣放入75%乙醇溶液中固定后,采用甘油明胶封片保存。结论可采用无味型封片剂或者甘油明胶代替中性树胶作为蠕形螨标本制作的封片剂。采用2%洗洁精可清洗标本中存在的油脂且可达到从透明胶带中游离蠕形螨的效果。

Objective To establish a safe and rapid preparation method for long-term preservation of human Demodex specimen,and to meet the needs of experimental teaching of human parasitology.Methods Human Demodex mites were obtained by the cellophane tape method and the squeezing method and were treated with various methods to compare the sealing effect of neutral balsam,odorless mounting medium,and glycerol jelly mounting medium,and the morphology of mite was observed and photographed under the KoPa WiFi EDU software system of the digital microscope.Results The specimens with few background impurities or little grease which did not affect observation were directly sealed with odorless mounting medium or glycerol jelly mounting medium.In contrast,the specimens with excessive bubbles,grease,or hair which affected observation were first cleaned with 2%dish soap for a short period of time,then placed in 75%-100%ethanol for dehydration,and finally sealed with odorless mounting medium.If there was a high density of mites on one sheet of adhesive tape,such specimens were first soaked in 2%dish soap for a long time and then centrifuged,and the mites collected were fixed with 75%ethanol and sealed with a glycerol jelly mounting medium.Conclusion Odorless mounting medium or glycerol jelly mounting medium can replace neutral balsam as the mounting medium for preparing Demodex specimen,and 2%dish soap can remove the excess grease in specimen and easily release the mites from the adhesive tape.