体外消化对油茶蒲提取物抑制α-葡萄糖苷酶活性的影响

Effect of In Vitro Digestion on the Inhibitory Activity of Oiltea Camellia Extract onα-Glucosidase

为探索体外消化对油茶蒲活性成分的影响,本试验以油茶蒲为原料,分析消化前后油茶蒲提取物、水相、正丁醇相、乙酸乙酯相对α-葡萄糖苷酶抑制活性的变化,采用高效液相色谱(HPLC)、分子对接进一步探索油茶蒲中抑制α-葡萄糖苷酶的主要活性物质。结果表明,油茶蒲提取物、乙酸乙酯相、正丁醇相、水相对α-葡萄糖苷酶的IC_(50)为0.27~1.17μg·mL^(-1),经体外模拟消化后,IC_(50)上升至0.34~2.36μg·mL^(-1),其中乙酸乙酯相的活性最强。油茶蒲提取物成分分析结果显示,体外消化能影响各相萃取物的总酚含量,其中乙酸乙酯相的总酚含量最高(44.49μg·mL^(-1))且在体外消化中具有良好的稳定性。相关性分析结果表明,油茶蒲提取物中有5个化合物与α-葡萄糖苷酶的IC_(50)呈显著相关,其中包括3-O-甲基鞣花酸-4'-O-β-D-吡喃葡萄糖苷(MEAG)。AutoDock分子对接结果也表明,MEAG具有良好的α-葡萄糖苷酶抑制活性,以及最佳的结合能(-7.99 kcal·mol^(-1))和估计抑制常数(1.40μmol·L^(-1)),其主要通过氢键和疏水作用与α-葡萄糖苷酶的氨基酸残基相互作用。本研究结果为开发新型α-葡萄糖苷酶抑制剂以及油茶蒲资源利用提供了数据参考。

In order to explore the effects of digestion on the active components of oiltea camellia in vitro,Oiltea Camellia fruit hull was used as the raw material,α-glucosidase inhibitory activity of oiltea camellia extract,aqueous phase,n-butanol phase and ethyl acetate phase were analyzed during in vitro digestion.Furthermore,high performance liquid chromatography(HPLC)and molecular docking were used to explore theα-glucosidase inhibitors in oiltea camellia.The results showed that the IC_(50)values of oiltea camellia extracts,ethyl acetate phase,n-butanol phase and water phase onα-glucosidase were 0.27~1.17μg·mL^(-1).After simulated digestion in vitro,the IC_(50)values increased to 0.34~2.36μg·mL^(-1),and the inhibitory activity of ethyl acetate phase was the strongest.Composition analysis showed that in vitro digestion could affect the total phenol content of the extracts,and the ethyl acetate phase had the highest total phenol content(44.49μg·mL^(-1))and had good stability during digestion.Composition-activity analysis showed that five compounds in oiltea camellia extracts were correlated with the IC_(50)values ofα-glucosidase,including 3-O-methylellagic acid-4'-O-β-D-glucopyranoside(MEAG).The results of AutoDock molecular docking also showed that MEAG had a goodα-glucosidase inhibitory activity,with the best binding energy of-7.99 kcal·mol^(-1)and estimated inhibition constant of 1.40μmol·L^(-1),which interacted with the amino acid residues ofα-glucosidase through hydrogen bond force and hydrophobic forces.This study provided a theoretical basis for the development of newα-glucosidase inhibitors and utilization of Oiltea Camellia.