pH 调控临床光滑念珠菌生长的机制

Regulatory mechanism of pH on the growth of clinical Candida glabrata

光滑念珠菌Candida glabrata是仅次于白念珠菌C.albicans的第二大念珠菌病原体,在免疫缺陷人体中引发较高的发病率和死亡率。该物种能在复杂多变的宿主环境中生存,pH是宿主体内重要的环境因子,光滑念珠菌适应不同生态位中pH的能力与其致病性密切相关。本研究发现,pH能够影响光滑念珠菌细胞增殖,尤其在相对碱性的pH环境中,光滑念珠菌的生长受到显著抑制。进一步研究发现,pH感应途径关键调控因子Rim101、Rim8和Rim21在其中发挥重要调控作用,并介导TOR信号通路调控细胞增殖。同时发现碱性pH环境能够破坏光滑念珠菌细胞壁完整性。上述结果表明,碱性pH环境影响光滑念珠菌生长的抑制及细胞壁完整性,本研究可为该菌临床感染的防控提供参考。

Candida glabrata is the most frequently isolated pathogen of candidiasis in immunocompromised hosts next to C.albicans.It is survivable under a wide range of environmental conditions,and the ability to adapt to pH changes in the host niches is closely related to pathogenesis.This study found that alkaline pH conditions repressed cell proliferation of C.glabrata,and the key regulators Rim101,Rim8,and Rim21 were involved in this regulation.The TOR signaling pathway plays an important role in the control of pH-regulated cell proliferation.It was further found that alkaline pH also affected the cell wall integrity of C.glabrata.Facts draw the conclusion that the inhibitory effects of pH changes on cell growth and cell wall integrity could provide clues to the development of new strategies of the control of infections caused by C.glabrata.