香菇菌丝后熟转色的转录组分析

Transcriptome analysis of Lentinula edodes during brown mycelium-film formation

香菇是世界第二大栽培食用菌,其菌丝后熟转色是其特有的栽培过程,并对其产量和品质至关重要。为了进一步了解香菇后熟转色形成的机制,本研究利用Illumina二代高通量测序平台,对初始未转色菌丝(LE118)、光照下转色菌丝(LE313C)、黑暗下未转色菌丝(LE313W)进行转录组测序,以香菇单核体W1-26基因组为参考基因组进行转录组有参分析,共鉴定出2215个差异表达基因,LE313C比LE118、LE313C比LE313W、LE313W比LE118中分别有1216、1507、1054个基因差异表达。GO富集分析表明,差异表达基因显著富集碳水化合物代谢过程、氧化还原酶活性、水解酶活性、过氧化物酶活性等。KEGG分析发现芳香族化合物的降解在LE313C比LE313W中被显著富集。在LE313C比LE118即香菇菌丝后熟转色发育过程中,筛选出关键差异表达基因包括糖苷酶、疏水蛋白、P450、漆酶、谷氨酸脱氢酶等,表明这些差异基因在香菇菌丝的后熟转色中可能行使重要的生物功能。本研究为进一步研究香菇菌丝后熟及转色形成的机理提供了重要依据。

Lentinula edodes ranks the second among cultivated edible mushrooms in the world.The formation of brown film(BF)on the mycelial tissue surface is a feature of sawdust cultivation for L.edodes,and this process is critical for ensuring high quantity and quality of fruiting body formation.To understand the molecular mechanisms underlying this process in L.edodes,the high-throughput Illumina was used to analyze the transcriptome data of initial white mycelial sample(LE118),brown mycelial film sample formed under light inducement(LE313C)and white mycelial sample formed in dark(LE313W).The genome of L.edodes W1-26 was used as a reference for transcriptome analysis.In total,2215 differentially expressed genes(DEGs)were identified.Of which,1216,1507 and 1054 genes were differentially expressed in LE313C/LE118,LE313C/LE313W and LE313W/LE118,respectively.GO enrichment analysis showed that the DEGs were significantly enriched in carbohydrate metabolism process,oxidoreductase activity,hydrolase activity,peroxidase activity,etc.KEGG analysis showed that the degradation of aromatic compounds was significantly enriched in LE313C/LE313W.In the process of BF formation in L.edodes(LE118 to LE313C),the key differentially expressed genes such as glycoside hydrolase,hydrophobins,P450,laccase,glutamate dehydrogenase were screened,suggesting important biological functions of these DEGs.This study provides an important basis for further research on the mechanism of BF brown formation in L.edodes.