超声辅助碱溶酸沉法提取鸡腿菇蛋白质工艺优化

Optimization of Ultrasonic-Assisted Alkali-Solution-Acid Sedimentation Method for Extraction of Proteins from Coprinus coprinatus

采用响应面法优化鸡腿菇蛋白质提取工艺,并研究最佳工艺条件下所得的鸡腿菇蛋白质的纯度。采用超声波辅助法提取鸡腿菇蛋白质,通过单因素试验研究pH、超声频率、提取时间和提取温度对鸡腿菇蛋白质得率和蛋白质纯度的影响。在单因素试验的基础上,利用响应面分析法优化超声波辅助提取鸡腿菇蛋白质的最佳工艺,并确定最佳工艺条件下鸡腿菇蛋白质的得率及纯度。超声波辅助碱溶酸沉法提取鸡腿菇蛋白质的最佳工艺为p H 12.6、超声功率125 W、提取时间66 min、提取温度42℃。在优化的最佳工艺条件下,鸡腿菇蛋白质得率为32.02%±0.35%,相比较传统碱溶酸沉法提取的鸡腿菇蛋白质,试验所用方法使蛋白质提取率提高7.72%,且鸡腿菇蛋白质纯度为84.5%。

Response surface methodology(RSM) was used to optimize the extraction process of Coprinus comitatus protein and to study the purity of Coprinus comitatus protein obtained under the optimal condition.The effects of pH,ultrasonic frequency,extraction time and extraction temperature on the protein yield and protein purity of Coprinus comitatus were studied by single factor experiments.On the basis of single factor experiment,response surface analysis(RSM) was used to optimize the ultrasonic-assisted extraction process of Coprinus comitatus protein,and to determine the yield and purity of Coprinus comitatus protein under the optimal conditions.The optimal extraction condition was as follows:pH 12.6,ultrasonic power 125 W,extraction time 66 min and extraction temperature 42 ℃.Under the optimal condition,the protein yield of Coprinus comitatus was 32.02%±0.35%.Compared with the traditional method of alkali-solution-acid precipitation,the protein extraction yield of Coprinus comitatus was increased by 7.72%,and the protein purity of Coprinus comitatus was 84.5%.