摘要
以凌云白毫为原料,在单因素试验结果的基础上,采用响应面法优化其总酚的超声波辅助提取工艺,并研究总酚的抗氧化活性。经试验论证获得提取工艺的最优条件:乙醇体积分数78%,超声时间21 min,提取温度81℃,液料比46∶1 (mL/g),超声功率160 W。在此条件下实测总酚提取率为19.26%。抗氧化活性试验证实,总酚对·OH及DPPH自由基具有良好的清除效果,且其清除能力与总酚质量浓度呈正向关系。该试验结果为凌云白毫的推广提供数据支撑。
It was to explore the optimal conditions of ultrasonic wave-assisted extraction of total phenols in Lingyun Pekoe tea and to evaluate its antioxidant activity.Based on the results of single factor experiments,the Box-Behnken method was used to optimize the extraction process,and the results were obtained as follows:ethanol concentration 78%,ultrasonic time 21 min,temperature 81 ℃,liquid-material ratio 46∶1(mL/g) and ultrasonic power 160 W.Under the optimal conditions,an average extraction yield of 19.26% was obtained.Investigation on antioxidant activity indicated that the polyphenols in Lingyun Pokoe tea had a good scavenging activity on ·OH and DPPH radical,which would be stronger with higher concentration.The results mentioned above provided supporting data for marketing Lingyun Pekoe in domestic and international.
作者
冼丽清
李珊
冯彬
梁俭
刘晓凤
XIAN Liqing;LI Shan;FENG Bin;LIANG Jian;LIU Xiaofeng(Key Laboratory of Regional Ecological Environment Analysis and Pollution Control of West Guangxi,School of Chemistry and Environment Engineering,Baise University,Baise 533000;School of Materials Science and Engineering,Baise University,Baise 533000)
出处
《食品工业》
CAS
2022年第1期57-61,共5页
The Food Industry
基金
国家自然科学基金项目(编号:21901009)
广西自然科学基金项目(编号:2017GXNSFBA198225)
广西高校中青年教师基础能力提升项目(编号:2017KY0719)
广西高校重点学科-材料物理与化学(编号:080501)。
关键词
凌云白毫
总酚
超声波辅助提取
响应面优化
抗氧化活性
Lingyun Pekoe tea
total phenols
ultrasonic wave-assisted extraction
response surface optimization
antioxidant activity
