2019—2020年浙江省湖州市城区白纹伊蚊成蚊杀虫剂耐受性的比较

Insecticide resistance of adult Aedes albopictus in Huzhou City of Zhejiang Province from 2019 to 2020

【目的】观察2019—2020年湖州市城区的白纹伊蚊成蚊对常用杀虫剂的抗性水平,比较白纹伊蚊抗性水平的变化情况。【方法】2019年和2020年在湖州市城区不同方位居民区采集幼蚊,饲养至成蚊,采用世界卫生组织(WHO)推荐的成蚊接触筒法进行5种杀虫剂抗药性测定。将诊断药纸杀虫剂剂量分为低含量和高含量,比较自然种群对常用杀虫剂抗性水平的变化。【结果】2019年对0.40%高效氯氰菊酯、0.10%溴氰菊酯、0.50%马拉硫磷、0.05%残杀威和3.00%氯菊酯的死亡率范围为97.06%~100.00%。2020年对0.40%高效氯氰菊酯、0.08%高效氯氰菊酯、0.03%溴氰菊酯、0.40%氯菊酯、0.50%马拉硫磷和0.02%残杀威的校正死亡率分别为98.58%、89.09%、85.64%、91.34%、91.43%和100.00%。当诊断药纸杀虫剂为高含量,自然种群对高效氯氰菊酯、溴氰菊酯、马拉硫磷和残杀威均为敏感种群,对氯菊酯表现为可能抗药性;当诊断药纸杀虫剂为低含量,自然种群对高效氯氰菊酯、溴氰菊酯、氯菊酯和马拉硫磷均为可能抗药性,对残杀威表现为敏感种群。【结论】2019—2020年湖州市城区白纹伊蚊对残杀威仍敏感,而对马拉硫磷发生变化,从敏感到产生一定程度抗药性。

[Objective]To investigate the resistance level of adult Aedes albopictus to commonly used insecticides in urban areas of Huzhou City,and analyze the trend of insecticide resistance.[Methods]Aedes albopictus larvae were collected from different urban areas of Huzhou City in 2019 and 2020. The larvae were reared to adults for determining resistance using the WHO standard test kits by contact tube method.[Results]The mortality was 97.06%-100.00% in adult Aedes albopictus mosquitoes exposed to 0.4% beta-cypermethrin,0.1% deltamethrin,0.5% malathion,0.05% propoxur and 3% permethrin in 2019. While the adult mosquitoes exposed to 0.4% beta Cypermethrin,0.08% beta Cypermethrin,0.03% deltamethrin,0.4% permethrin,0.5% malathion and 0.02% propoxur in 2020,the adjusted mortality were 98.58%,89.09%,85.64%,91.34%,91.43% and 100% respectively. When the insecticide doses on medicated paper were high,the Huzhou populations of Aedes albopictus were sensitive to beta-cypermethrin,deltamethrin,malathion and propoxur,and showed suspected resistance to permethrin.While the Aedes albopictus were exposed to low testing dose,they showed suspected resistance to beta-cypermethrin,deltamethrin,permethrin and malathion,but remained to be sensitive to propoxur.[Conclusion]The adult Aedes albopictus in Huzhou City from 2019 to 2020 was still sensitive to propoxur,but their resistance to malathion changes from sensitive to resistant. When the contact tube method is used for insecticide resistance,it is suggested that the accurate diagnostic dose should be obtained first before conducting further insecticide resistance tests.