体外模拟消化对桑葚酸奶酚类化合物稳定性和抗氧化活性的影响

Effects of Simulated Digestion in Vitro on Stability and Antioxidant Activity of Mulberry Yogurt Phenol Compounds

为探究消化对桑葚酸奶中酚类物质稳定性及抗氧化活性的影响,建立口腔、胃、肠体外消化模型,以总多酚、总黄酮含量为多酚类物质稳定性指标,以总抗氧化活力、ABTS+•自由基清除能力和DPPH•自由基清除能力为抗氧化指标,测定消化处理前后各指标变化特征。结果表明:模拟口腔消化对各样品的酚类物质和抗氧化活性物质无显著影响(P>0.05);模拟胃消化过程中试验组各样品的总多酚含量、总黄酮含量、总抗氧化、ABTS+•自由基清除能力及DPPH•自由基清除能力均显著提升(P<0.05);模拟肠消化过程中试验组各样品的酚类物质与抗氧化活性物质含量均高于消化前和对照组含量,但总多酚含量、总黄酮含量和总抗氧化活力在肠消化过程中均呈现先升高后下降的变化趋势,ABTS+•自由基清除能力及DPPH•自由基清除能力在肠消化过程中均呈现持续下降的变化;相关性分析显示,桑葚酸奶中总多酚含量和总黄酮含量、总抗氧化活力呈极显著相关性(P<0.01),相关系数分别为0.962和0.891。

In order to explore the influence of digestion on phenolic stability and antioxidant activity in mulberry yogurt,the oral,stomach and intestinal digestive models in vitro were established.Total phenol and flavonoids content were used as the stability index of polyphenols,and the total antioxidant vitality,ABTS+•free radical clearance ability and DPPH•radical clearance ability were used as antioxidant index.The indicator characteristics were determined before and after digestive processing.The results showed that the simulated oral digestion had no significant effect on the phenolic and antioxidant active substances(P>0.05).The total polyphenols content,total flavonoids content,total antioxidant content,ABTS+•free radical clearance ability and DPPH•free radical clearance ability were significantly improved during the simulated gastric digestion(P<0.05).The content of phenolic and antioxidant active substances in the experimental group was higher than those before digestion and control groups.However,total phenol content,total flavonoids content and total antioxidant vitality increased first and then decreased during intestinal digestion.ABTS+•radical clearance and DPPH•radical clearance decreased continuously during intestinal digestion.Correlation analysis showed that the total polyphenols content and the total flavonoids content in mulberry yogurt was significantly correlated with the total antioxidant vitality(P<0.01),and the correlation coefficients were 0.962 and 0.891,respectively.