两种固定液在小鼠睾丸组织HE及免疫组织化学染色中的固定效果比较

Comparison of fixation effects of two fixation solutions in H&E and immunohistochemical staining of mouse testicular tissue

目的比较两种固定液对小鼠睾丸组织石蜡切片和冰冻切片质量的影响,优化出小鼠睾丸组织最适宜的固定方法。方法取8只C57小鼠,随机分为2组,每组4只,将睾丸组织取出后分别置于modified Davidson Fluid(mDF)固定液和4%多聚甲醛(4%PFD)溶液,固定24 h后进行石蜡和冰冻切片,随后通过HE染色和免疫组织化学染色分析比较两种固定液对睾丸组织结构的固定效果及抗原保护作用。结果与4%PFD溶液组相比,mDF固定液组睾丸组织结构完整清晰,间质成分无脱落,对抗原保护作用较强。结论mDF固定液是较好保存睾丸组织形态结构的固定液。

Objective To compare the effect between two kinds of fixation solution on the quality of paraffin section and frozen section,and to optimize the best fixation method for mouse testicular tissue.Methods 8 mice were randomLy divided into 2 groups,4 mice for each group.After the testis tissue were removed,they were placed in modified Davidson Fluid(mDF)and 4%paraformaldehyde(4%PFD)solution,respectively.After 24 h of fixation,paraffin and frozen sections were performed.Then,HE staining and immunohistochemical staining were used to analyze and compare the fixation effect and antigenic protection of the two kinds of fixation solution.Results Compared with the 4%PFD group,the testicular tissue structure of mDF group was intact and clear,and the interstitial did not fall off,and the a stronger antigen protection effect of mDF fixation solution group was observed.Conclusion mDF fixation solution is a better fixative to preserve the morphological structure of mouse testicular tissue.