穿心莲对斑点叉尾鮰消化酶活性的影响

Effect of Andrographis paniculata on digestive enzyme activities of channel catfish(Ictalurus punctatus)

为研究穿心莲(Andrographis paniculata)对斑点叉尾鮰(Ictalurus punctatus)消化酶活性的影响,试验选取平均体重为(5.92±0.63)g的健康斑点叉尾鮰270尾,随机分成9组(G0、G1、G2、G3、G4、G5、G6、G7、G8组),每组设3个重复,每个重复10尾鱼,每组分别投喂添加不同比例穿心莲粉(0%、0.38%、0.75%、1.13%、1.50%、1.88%、2.25%、2.63%、3.00%)的饲料。饲养试验时间为30 d。结果发现,肠道中的消化酶,G0和G1组的脂肪酶活性显著高于其它各组(P<0.05);G8组的淀粉酶活性最高,显著高于G0、G1、G4和G7组(P<0.05);胰蛋白酶活性总体呈现出先上升后下降的趋势,以G6组的最高。结果表明,在斑点叉尾鮰饲料中添加1.88%~2.25%的穿心莲能提升胰蛋白酶的活性。

To understand the effects of Andrographis paniculata on digestive enzyme activities of channel catfish(Ictalurus punctatus),I.punctatus(average initial body weight 5.92 g±0.63 g)were divided into 9 treatment groups(G0,G1,G2,G3,G4,G5,G6,G7,G8)with 3 replicates,fed for 30 days with nine experimental diets.The results showed that,the lipase activities in guts of group G0 and G1 were significantly higher than other groups(P<0.05).The amylase activities in guts of group G8 were the highest,which were significantly higher than group G0,G1,G4 and G7(P<0.05).The trypsase activities in guts showed a trend of rising first and then falling.The trypsase activities of group G6 were the highest.The results indicated that the diets supplemented with Andrographis paniculata at 1.88%~2.25%adding level could enhance the trypsin activities.