HPV E6/E7 mRNA与p16/Ki-67双染检测的关系及对高危型人乳头瘤病毒感染引起宫颈病变的检出能力评估

Relationship between HPV E6/E7 mRNA and p16/Ki-67 dual staining detection and evaluation on the detection ability of cervical lesions caused by high-risk human papillomavirus infection

目的探究人乳头瘤病毒(HPV)E6/E7 mRNA与p16/Ki-67双染检测的关系及对高危型HPV感染引起宫颈病变的检出能力。方法选取2020年1月至2021年4月高州市人民医院收治的200例高危型HPV感染患者,均接受病理组织检查。统计病理组织、细胞学、HPV E6/E7 mRNA与p16/Ki-67双染检测阳性细胞学检查结果,比较不同细胞学、病理学类型患者HPV E6/E7 mRNA与p16/Ki-67双染检测阳性率,不同HPV E6/E7 mRNA表达情况患者p16/Ki-67双染检测阳性率,分析HPV E6/E7 mRNA与p16/Ki-67双染检测的关系,并以病理组织检查为“金标准”,统计HPV E6/E7 mRNA与p16/Ki-67双染检测及联合检查的诊断效能。结果随细胞学、病理学诊断级别的提升,HPV E6/E7 mRNA、p16/Ki-67双染检测阳性率逐渐增加,差异有统计学意义(P<0.05);HPV E6/E7 mRNA阳性患者的体重指数、p16/Ki-67双染检测阳性率高于HPV E6/E7 mRNA阴性患者,差异有统计学意义(P<0.05);p16/Ki-67双染检测阳性为HPV E6/E7 mRNA阳性的危险因素;联合检查诊断CINⅡ+敏感度高于HPV E6/E7 mRNA、p16/Ki-67双染检测单独诊断,差异有统计学意义(P<0.05)。结论HPV E6/E7 mRNA、p16/Ki-67双染检测均可用于宫颈癌前病变筛查,联合应用可进一步提升对CINⅡ+的诊断价值,为临床诊断提供可靠依据。

Objective To explore the relationship between human papillomavirus(HPV)E6/E7 mRNA and p16/Ki-67 dual staining detection and their detection ability of cervical lesions caused by high-risk HPV infection.Methods A total of 200 patients with high-risk HPV infection treated in the People’s Hospital of Gaozhou from January 2020 to April 2021 were selected for histopathological examination.The results of pathological tissue,cytology,and the positive cytology results for HPV E6/E7 mRNA and p16/Ki-67 dual staining were counted,and the positive rates of HPV E6/E7 mRNA and p16/Ki-67 dual staining in patients with different cytology and pathology results and the positive rates of p16/Ki-67 dual staining in patients with different HPV E6/E7 mRNA expression were compared.The relationship between HPV E6/E7 mRNA and p16/Ki-67 was analyzed,the histopathological examination was taken as the"gold standard",and the diagnostic efficacy of HPV E6/E7 mRNA alone,p16/Ki-67 dual staining alone and combined examination was counted.Results The positive rates of HPV E6/E7 mRNA and p16/Ki-67 dual staining increased gradually with the improvement of cytological and pathological diagnosis levels,with statistically significant differences(P<0.05).The body mass index and positive rate of p16/Ki-67 dual staining in patients with HPV E6/E7 mRNA positive was higher than that in patients with HPV E6/E7 mRNA negative,with statistically significant difference(P<0.05).The positive p16/Ki-67 dual staining was a risk factor for HPV E6/E7 mRNA positive expression.The sensitivity of combined examination in the diagnosis of CINⅡ+was higher than that of HPV E6/E7 mRNA and p16/Ki-67 alone,with statistically significant difference(P<0.05).Conclusion HPV E 6/E7 mRNA a nd p16/Ki-67 dual staining can be used for cervical precancerous lesion screening.The combined application can further improve the diagnostic value of CINⅡ+,and provide a reliable basis for clinical diagnosis.