APOE、Resistin、miR-24在老年动脉粥样硬化性脑梗死患者中的表达及对预后的预测价值

Expression of APOE,resistin and miR-24 in elderly patients with ACI and its predictive value

目的探讨载脂蛋白E(Apolipoprotein E,APOE)、抵抗素(Resistin)、微小RNA-24(MicroRNA-24,miR-24在老年动脉粥样硬化性脑梗死(ACI)患者中的表达及与病情严重程度相关性。方法选取2020年8月~2021年8月于我院进行治疗的老年ACI患者80例为ACI组,选取同期于我院进行诊治的无症状性颈动脉狭窄(ACS)患者80例为ACS组,另选取同期于我院行健康体检的志愿者80例为对照组。评价患者斑块、血管狭窄程度。免疫透射比浊法检测APOE水平,酶标分析仪检测Resistin水平,RT-PCR法检测miR-24表达,分析APOE、Resistin、miR-24在老年ACI患者中的表达及对患者预后的预测价值。结果与对照组比较,ACS组、ACI组APOE、Resistin、miR-24表达水平较高;ACI组患者APOE、Resistin、miR-24表达水平高于ACS组(P<0.05)。与无斑块患者比较,有斑块患者APOE、Resistin、mi R-24表达水平较高(P<0.05);与稳定性斑块、轻中度血管狭窄、预后良好患者比较,不稳定性斑块、重度血管狭窄、预后不良患者APOE、Resistin、miR-24表达水平较高(P<0.05)。ACI患者APOE和Resistin、APOE和miR-24、Resistin和mi R-24均呈正相关(r=0.465,0.641,0.467,P=0.001、0.001、0.001)。与APOE、Resistin、miR-24单项检测相比,三项联合检测对ACI患者预后预测的灵敏度较高,特异性较低(P<0.05)。结论老年ACI患者APOE、Resistin、miR-24表达水平明显异常,三者表达水平与患者临床特征密切相关,且三者具有一定相关性,联合检测APOE、Resistin、miR-24对老年ACI患者预后具有一定的预测价值。

Objective To explore the expression of apolipoprotein E(APOE),resistin and microRNA-24(miR-24)in elderly patients with atherosclerotic cerebral infarction(ACI)and its correlation with the severity of the disease.Methods Eighty elderly patients with ACI who were treated in our hospital from Aug 2020 to Aug 2021 were selected as the ACI group,and 80 patients with asymptomatic carotid stenosis(ACS)who were diagnosed and treated in our hospital during the same period were selected as the ACS group,and 80 volunteers who underwent healthy body examination in our hospital during the same period were also selected as the control group.Evaluated the plaque and degree of vessel stenosis in patients,APOE levels were detected by immunotransmission turbidimetry,resistin levels were detected by microplate analyzer,and miR-24 expression was detected by RT-PCR assay,the expression of APOE,resistin,and miR-24 were analyzed in elderly patients with ACI and their prognostic value for patients outcomes.Results The expression levels of APOE,resistin and miR-24 in ACS and ACI groups were higher than those in control group.The expression levels of APOE,resistin and miR-24 in ACI group were higher than those in ACS group(P<0.05).The levels of APOE,resistin and miR-24 in patients with plaque were higher than those in patients without plaque(P<0.05).Compared with patients with stable plaque,mild and moderate vascular stenosis and good prognosis,patients with unstable plaque,severe vascular stenosis and poor prognosis had higher expression levels of APOE,resistin and miR-24(P<0.05).The expression levels of APOE and resistin,APOE and miR-24,resistin and miR-24 were positively correlated in patients with ACI(r=0.465,0.641,0.467,P=0.001,0.001,0.001).Compared with the single tests of APOE,resistin and miR-24,the three combined tests had higher sensitivity and lower specificity in predicting the prognosis of patients with ACI(P<0.05).Conclusion The expression levels of APOE,resistin and miR-24 in elderly patients with ACI are significantly abnormal and closely correlated with the clinical characteristics of the patients,and the three have a certain correlation.The combined detection of APOE,resistin and miR-24 have certain predictive value for the prognosis of senile patients with ACI.