基于网络药理学初步探索散偏汤治疗偏头痛的作用机制

Preliminary Study on the Mechanism of Sanpian Decoction in the Treatment of Migraine Based on Network Pharmacology

目的:采用网络药理学方法初步探索散偏汤治疗偏头痛的作用机制。方法:利用TCMSP数据库收集散偏汤各药物的成分,进行ADME筛选,得到潜在活性成分,并利用SwissTargetPrediction获取活性成分的作用靶点;运用OMIM数据库和GeneCards数据库搜集偏头痛相关靶点,将药物靶点与疾病靶点导入jvenn网站,获取共有靶点;使用STRING数据库构建共有靶点PPI网络,运用Cytoscape3.7.2软件构建"药物-靶点-疾病"网络图,并对该网络进行分析;将共有靶点导入Metascape数据库对其进行KEGG通路富集分析和GO富集分析。结果:筛选得到205个共同靶点,PPI分析得到25个关键基因。"药物-靶点-疾病"网络图包含化合物157个,靶点205个。KEGG通路富集筛选得到256条信号通路(p<0.01)。GO功能富集分析得到GO条目550个(p<0.01),其中包括BP条目306个、CC条目108个、MF条目136个。结论:散偏汤治疗偏头痛的潜在活性成分可能是山柰酚、罗通定、独活素等,且与APP、CCR5、OPRK1、CXCL8等基因相关性较大。散偏汤可能通过调控MAPK、PI3K-Akt、HIF-1等信号通路对偏头痛发挥治疗作用。

Objective:The network pharmacology method was used to explore the mechanism of Sanpian Decoction(SPD)in the treatment of migraine.Methods:TCMSP database was used to collect the main components of drugs in SPD.At the same time,ADME screening was carried out to obtain the potential active components.Meanwhile,SwissTargetPrediction was used to obtain the function targets of active components.OMIM database and GeneCards database were used to collect migraine-related targets,and importing the screened drug targets and disease targets into the jvenn website to acquire common targets.The PPI network of common targets was constructed by using STRING database,and the“drug target disease”network diagram was constructed by using Cytoscape 3.7.2 software.Then the network was analyzed.The common targets were imported into Metascape database in order to analyze the GO enrichment and KEGG pathway enrichment.Results:There were 205 common targets by filtering and 25 key genes were identified by PPI analysis.The“drug-target-disease”network contains 157 compounds and 205 targets.256 signaling pathways were gained by KEGG pathway enrichment.GO enrichment analysis showed 550 GO items(p<0.01),which includes 306 biological process items,108 cell component items,and 136 molecular function items.Conclusion:The potential active components of SPD for the treatment of migraine may be Kaempferol,Rotundine and Heraclenin,which are closely related to genes such as APP,CCR5,OPRK1,CXCL8.SPD may play a therapeutic role in migraine by regulating MAPK signaling pathway,PI3 K-Akt signaling pathway,and HIF-1 signaling pathway.