微波对固体培养基灭菌的研究

Research on Sterilization of Solid Medium by Microwave

自新冠肺炎疫情爆发以来,各行各业对消毒灭菌的要求越来越高,因此开展微波灭菌技术的研究意义重大。传统的微波灭菌技术中由于固体培养基存在受热不均匀的问题,导致出现灭菌"冷点",灭菌时间过长。文章首先对微波辐射腔的电场分布特性和固体培养基的温度分布特性进行了仿真研究。研究结果表明:微波辐射腔内的电场分布呈现馈源较近区域内场强大于较远区域内场强的特点,固体培养基的温度呈现出四周高于中心位置的特征。然后采用在辐射腔内添加搅拌器的措施,有效改善了腔体内部的电场分布和培养基的温度分布特性。最后通过微波灭菌试验证明了搅拌器能显著改善微波灭菌中的受热不均匀问题,提高了灭菌率。

Since the outbreak of novel coronavirus pneumonia, the requirements for disinfection and sterilization have been increasing in all walks of life. Therefore, it is of great significance to research the technology of microwave sterilization. In the traditional microwave sterilization technology, due to the uneven heating of solid medium, the sterilization “cold point” appears and the sterilization time is too long. Firstly, the electric field distribution characteristics of the microwave radiation cavity and the temperature distribution characteristics of the solid medium are simulated. The results indicate that the electric field distribution in the microwave radiation cavity shows that the electric field near the source field is greater than that far away, and the temperature of the solid medium is higher around and lower in the middle. Then, a stirrer is added into the radiation chamber to improve the electric field distribution and the temperature distribution of the medium. Finally, through the microwave sterilization test, it is proved that the stirrer can significantly improve the uneven heating in microwave heating sterilization, and increase the sterilization rate.