白藜芦醇对高糖诱导的人脐静脉内皮细胞增殖、迁移及管腔形成的影响

Effects of resveratrol on proliferation,migration and tube formation of human umbilical vein endothelial cells in high-glucose environment

目的︰观察白藜芦醇(RSV)对高糖培养条件下人脐静脉内皮细胞(HUVEC)增殖、迁移和管腔形成的影响。方法﹐将体外培养的HUVEC细胞随机分为空白对照组、甘露醇对照组(30 mmolL甘露醇)模型组(30 mmol/L D-葡萄糖)和白藜芦醇低、中、高剂量组。30 mmolL高糖诱导,并用不同浓度的白藜芦醇(20,40,60 μmol/L)干预内皮细胞24 h,分别设置为白藜芦醇低、中、高剂量组。采用CCK-8法检测细胞增殖,Transwell小室及划痕实验检测细胞迁移,基质胶(Matrigel)法检测细胞管腔形成能力。结果﹑模型组的细胞增殖率、迁移数,划痕愈合率及管腔形成分支点数均明显高于空白对照组及甘露醇对照组(P<0.05)。与模型组相比,白藜芦醇干预后,高糖诱导的HU-VEC细胞成管作用减弱,白藜芦醇低、中﹑高剂量组细胞增殖率、迁移数﹑划痕愈合率及管腔形成分支点数均明显低于模型组(P<0.05),且与白藜芦醇浓度呈剂量依赖性,当白藜芦醇浓度为60 μmol/L时,HUVEC细胞的增殖率,迁移数﹑划痕愈合率及管腔形成分支点数不仅低于模型组,且低于空白对照组和甘露醇对照组,差异具有统计学意义(P<0.05)。结论﹐白藜芦醇可以抑制高糖条件下HUVEC细胞增殖,迁移和管腔形成,提示白藜芦醇对高糖诱导的HUVEC细胞损伤具有一定的保护作用,可抑制病理刺激条件下血管生成。

Objective To observe the effects of resveratrol(RSV)on proliferation,migration and tube formation of human umbilical vein endothelial cells(HUVECs)in the context of high glucose.Methods Well-cultured HUVECs in vitro were randomly divided into six groups:blank group,mannitol 30 mmol/L group,model group,low-dose,mid-dose and high-dose RSV groups.The model group was induced by using 30 mmol/L D-glucose.The low-dose,mid-dose and high-dose RSV groups were established through intervention by administering 20 μmol/L,40 μmolL,60 μmol/L RSV respectively for 24 hours.Cell proliferation,migration and tube formation were detected by using Cell Counting Kit-8(CCK-8),Transwell chamber,wound healing and Matrigel assay respectively.Results The cell proliferation rate,migration number,wound healing rate and lumen branch number in model group were significantly higher than those in blank group and mannitol group(P<0.05).Compared with model group,after intervention with RSV,the ability of cell proliferation,migration and tube formation were significantly decreased.The cell proliferation rate,migration number,wound healing rate and lumen branch number were significantly decreased in a dose-dependent manner(P<0.05).When RSV concentration was 60 μmol/L,the proliferation rate,migration number,wound healing rate and lumen branch number were significantly lower than those in model group,blank group and mannitol group.The differences were statistically significant(P<0.05).Conclusion RSV could inhibit the proliferation,migration and lumen formation of HUVEC cells in high glucose conditions,suggesting that it could protect HUVEC cells from injury induced by high glucose and inhibit pathological angiogenesis.