高效液相色谱法检测蔬菜中甲萘威残留

Detection of Carbaryl Residue in Vegetables by High Performance Liquid Chromatography

本文采用高效液相色谱(HPLC)法检测叶类蔬菜(以菠菜为例)中甲萘威的残留。采用以十八烷基键合硅胶为固定相色谱柱,以甲醇-水=55∶45(V/V)为流动相,用二极管阵列检测器在268 nm波长下检测。菠菜样品采用超声波辅助提取进行前处理,提取液经浓缩后进行HPLC分析。该方法的线性范围为0.50~10.0 mg·kg^(-1)(R^(2)=0.9997),相对标准偏差为0.447%,加标回收率在82.1%~92.1%之间。经过对实际菠菜样品的检测,其甲萘威残留为0.882 mg·kg^(-1),未超过国家标准的限量规定。

This paper reported the detection of carbaryl residues in vegetables with high performance liquid chromatography(HPLC)via octadecyl bonded phase as stationary phase.The chromatographic conditions were determined and optimized using mobile phase of methyl alcohol and water(55∶45,V/V)and a diode array detector set at wavelength of 268 nm.The carbaryl was extracted from vegetable samples using ultrasonic extraction and centrifugation and then HPLC detection.The linear range of the method was 0.50-10.0 mg·kg^(-1)(R^(2)=0.9997),the relative standard deviation was 0.447%,and the recoveries were in the range of 82.1%-92.1%.In the carbaryl residues detection of actual spinach sample,carbaryl was 0.882 mg·kg^(-1),within the limit requirement of national standard.