莫西沙星及其杂质的NMR波谱研究

NMR spectra study on moxifloxacin and its impurities

目的:阐述莫西沙星及其杂质的NMR波谱特征。方法:应用一维及二维NMR技术对莫西沙星、8-羟基莫西沙星、8-氟基莫西沙星及8-乙氧基莫西沙星进行测试。结果:8位取代基对莫西沙星A,B,C,D,E环上质子的化学位移和耦合常数影响较小,同时对莫西沙星A,C,D,E环上碳原子的化学位移和耦合常数影响较小,但是对B环碳原子化学位移影响较大。结论:本研究总结了8位取代基改变对莫西沙星质子和碳原子化学位移和耦合常数的影响规律,为氟喹诺酮类抗生素的杂质结构鉴定提供了NMR谱化学位移参考。

Objective:To summarize the NMR spectra characteristics of moxifloxacin and its impurities.Methods:Moxifloxacin,8-hydroxy-moxifloxacin,8-fluoro-moxifloxacin and 8-ethoxymoxifloxacin are determined by one-dimensional and two-dimensional NMR.Results:The changes on the substituent group at position 8 have little effect on the chemical shift and coupling constant of protons on rings A,B,C,D and E of moxifloxacin,and on the chemical shift and coupling constant of carbon atoms on rings A,C,D and E of moxifloxacin,but has a great effect on the chemical shift and coupling constant of carbon atoms on the ring B.Conclusion:This study concluded that the influence pattern of substituent changes at 8-position on proton and carbon signals of moxifloxacin,providing NMR chemical shifts references for the impurity structure identification of fluoroquinolones.