番茄JAZ家族生物信息学及表达模式分析

Bioinformatics and Expression Pattern Analysis of the JAZ Gene Family in Tomato

为明确番茄JAZ家族成员的特征,本研究以番茄JAZ家族基因为研究对象,对该家族成员的进化、复制、理化性质、表达模式等进行分析。结果显示,SlJAZs主要分布在番茄的7条染色体上,SlJAZ9、SlJAZ10和SlJAZ11可能在Chr.8上形成串联重复事件。SlJAZs基因结构分析结果显示,内含子数目变异比较大,位于1 (SlJAZ2)~14 (Sl JAZ12)之间。启动子分析结果表明,13个SlJAZs基因启动子顺式作用元件种类和数量存在差异,推测其各自的功能也存在差异。组织表达模式分析表明,SlJAZs基因在番茄在根、茎、叶、花、果间存在不同的表达特性。其中,SlJAZ2、SlJAZ4、SlJAZ6、SlJAZ7、SlJAZ8、SlJAZ11、SlJAZ12、SlJAZ13在花中表达量相对较高;SlJAZ5在根中表达量相对较高;Sl JAZ10在叶片和果中表达相对较高;SlJAZ11在叶片中表达量相对较低,说明SlJAZs基因可能在番茄发育的各个阶段发挥重要作用,本研究为进一步鉴定番茄JAZ的功能提供了参考。

In order to clarify the characteristics of tomato JAZ family members, this study analyzed the evolution,replication, physical and chemical properties and expression patterns of JAZ family members in Solanum lycopersicum plants. The results showed that SlJAZs were distributed on seven chromosomes of tomato, and SlJAZ9,SlJAZ10 and SlJAZ11 might formed tandem repeats on Chr.8. SlJAZs gene structure analysis results showed that the number of introns varied from 1(SlJAZ2) to 14(SlJAZ12). Promoter analysis showed that there were differences in the types and numbers of cis acting elements in the 13 SlJAZs gene promoters, and it was speculated that their functions were also different. The analysis of tissue expression pattern showed that SlJAZs gene had different expression characteristics in root, stem, leaf, flower and fruit of tomato. Among them, the expression level of SlJAZ2, SlJAZ4, SlJAZ6, SlJAZ7, SlJAZ8, SlJAZ11, SlJAZ12, SlJAZ13 in flower was relatively high;the expression level of SlJAZ5 in root was relatively high;the expression level of SlJAZ10 in leaf and fruit was relatively high;the expression level of SlJAZ11 in leaf was relatively low. These results suggest that SlJAZs gene may play an important role in tomato development. This study provides a theoretical basis for further identifying the function of tomato JAZ.