枯草芽孢杆菌对拟南芥的干旱和盐胁迫耐受性的影响

Effects of Bacillus subtilis on Drought and Salt Stress Tolerance of Arabidopsis thaliana

为揭示枯草芽孢杆菌对拟南芥的干旱和盐胁迫耐受性的影响。本研究应用枯草芽孢杆菌QM3对拟南芥和大白菜进行培养,分别对植物进行干旱胁迫和盐胁迫处理,检测植物中干旱和盐胁迫诱导基因的表达,并测量气孔孔径及叶绿素含量。结果显示,干旱胁迫条件下,施用QM3后,拟南芥中4个干旱胁迫诱导基因(RD29B, RAB18, RD20和NCED3)被上调。盐胁迫条件下,QM3导致了拟南芥中盐胁迫标记基因(RD29B, SOS1, RD29A和WRKY8)的高表达。干旱胁迫后,与对照植物相比,经QM3处理的植物的存活率显著升高,而气孔孔径显著降低。此外,盐胁迫处理后,QM3促进了拟南芥的生长,并且升高了叶绿素水平。干旱胁迫后,QM3处理导致拟南芥的侧根数量和长度均增加。在干旱胁迫条件下,用QM3处理显著提高了大白菜的存活率。在盐胁迫条件下,用QM3处理显著提高了大白菜的总叶面积。QM3在干旱胁迫条件下促进了大白菜中干旱诱导基因如BrDREB1D、BrWRKY7和BraCSD3的高表达。本研究表明枯草芽孢杆菌菌株QM3显著增强了拟南芥和大白菜抗旱和盐胁迫的能力,并促进了植物生长。

In order to reveal the influence of Bacillus subtilis on the tolerance of Arabidopsis thaliana to drought and salt stress. In this study, Bacillus subtilis QM3 was used to cultivate Arabidopsis thaliana and Chinese cabbage,and then the plants were subjected to drought stress and salt stress, and then the expression of drought and salt stress-induced genes in the plants was detected, and the stomata diameter and chlorophyll content were measured.The results showed that under drought stress conditions, four drought-induced genes(RD29 B, RAB18, RD20 and NCED3) in Arabidopsis thaliana were up-regulated after QM3 was applied. Under salt stress conditions, QM3 resulted in high expression of salt stress marker genes(RD29 B, SOS1, RD29 A and WRKY8) in Arabidopsis thaliana.After drought stress, compared with control plants, the survival rate of plants treated with QM3 increased significantly, while the stomata diameter decreased significantly. In addition, after salt stress treatment, QM3 promoted the growth of Arabidopsis thaliana and increased the chlorophyll level. After drought stress, QM3 treatment resulted in an increase in the number and length of lateral roots of Arabidopsis thaliana. Under drought stress, treatment with QM3 significantly improved the survival rate of Chinese cabbage. Under salt stress, treatment with QM3 significantly increased the total leaf area of Chinese cabbage. QM3 promoted the high expression of drought-inducing genes such as BrDREB1 D, BrWRKY7 and BraCSD3 in Chinese cabbage under drought stress.This study showed that Bacillus subtilis strain QM3 significantly enhanced the ability of Arabidopsis thaliana and Chinese cabbage to resist drought and salt stress, and promoted plant growth.