不同激素配方、土壤基质对蒙古黄芪根系生长发育的影响

Effects of Different Hormone Formulations and Soil Matrix on Root Growth and Development of Astragalus mongolica

为选择出最适合黄芪增殖和生根的最优激素组合及移栽土壤基质,本研究通过正交试验探究了黄芪无菌苗在不同激素配方中的增殖情况、生根情况和移栽黄芪幼苗合适的土壤基质。研究表明,在茎段增殖培养阶段添加单一激素MS+6-BA 1.5 mg/L、添加两种激素MS+6-BA 1 mg/L+NAA 0.5 mg/L、添加三种激素MS+6-BA 2 mg/L+NAA 0.5 mg/L+IBA 0.2 mg/L为最佳组合;在生根培养中,最适合的激素组合为1/2MS+IAA2.0 mg/L+IBA 1.0 mg/L+NAA 1.5 mg/L;在移栽基质选择的研究中,低位泥炭∶蛭石∶珍珠岩=1∶1∶1/1∶2∶2的两种基质组合中的黄芪幼苗均长势优良。研究基质对黄芪根系生长发育的影响,旨在了解适合黄芪增殖、生根、移栽生长发育的基质激素配比,为黄芪的育种、栽培研究、大规模种植以及药用植物生物技术研究提供依据。

The purpose of this study is to select the most suitable hormone combination and transplanting soil matrix of Astragalus membranaceus seedlings. The results showed that in the stem segment proliferation culture,adding single hormone MS +6-BA 1.5 mg/L, adding two hormones MS +6-BA 1 mg/L +NAA 0.5 mg/L, adding three hormones MS+6-BA 2 mg/L+NAA 0.5 mg/L+IBA 0.2 mg/L were optimal combination. In the rooting culture,the most suitable hormone com-bination was 1/2 MS+IAA 2.0 mg/L+IBA 1.0 mg/L+NAA 1.5 mg/L. In the study of transplanting medium selection, Astragalus membranaceus seedlings in the two matrix combinations of low peat ∶Vermiculite ∶Perlite =1 ∶1 ∶1/1 ∶2 ∶2 showed good growth. To study the effect of substrate on the growth and development of Astragalus membranaceus root system, aiming to understand the ratio of substrate hormones suitable for Astragalus membranaceus, rooting, transplanting growth and development, so as to provide basis for Astragalus membranaceus breeding, cultivation research, large-scale planting and biotechnology research of medicinal plants.