虎杖HPLC指纹图谱及抗痛风性关节炎活性谱效关系研究

Study on relationship between HPLC fingerprint of Polygonum cuspidatum and active spectrum of anti gouty arthritis

目的建立虎杖Polygonumcuspidatum的HPLC指纹图谱,研究虎杖醇提物的HPLC指纹图谱与抗痛风性关节炎活性之间的谱效关系,初步明确虎杖抗痛风性关节炎主要活性成分。方法采用HPLC法建立虎杖的指纹图谱;使用"中药色谱指纹图谱相似度指纹评价系统(2004 A版)"进行相似度评价;以小鼠足垫厚度、血清尿酸含量为虎杖抗痛风性关节炎作用药效指标,采用灰色关联度分析法分析其谱效关系。结果虎杖发挥抗痛风性关节炎作用是多种成分共同作用的结果,对抗痛风性关节炎作用贡献较大的特征峰有7号峰(虎杖苷)、9号峰(槲皮素)、11号峰(白藜芦醇)、12号峰(槲皮苷)、13号峰(大黄素-8-O-β-D-葡萄糖苷)、16号峰(大黄素)、18号峰(大黄素甲醚)。结论虎杖发挥抗痛风性关节炎作用的药效是多种成分共同作用的结果,可为该药材质量控制与应用开发提供参考。

Objective To establish the HPLC fingerprint of Polygonum cuspidatum and to study the relationship between the HPLC fingerprint of P. cuspidatum alcohol extract and its anti gouty arthritis activity. Methods HPLC method was used to establish the fingerprint of P. cuspidatum;The “Fingerprint Similarity Evaluation System of Traditional Chinese Medicine Chromatogram(2004 A version)” was used to evaluate the similarity;The thickness of mouse foot pad and the content of serum uric acid were used as the pharmacodynamic indexes of P. cuspidatum in anti gouty arthritis, and the relationship between the spectrum and effect was analyzed by grey correlation analysis. Results The anti gouty arthritis effect of P. cuspidatum was the result of the joint action of many components.The characteristic peaks that contributed a lot to the anti gouty arthritis effect were No.7 peak(polydatin), No.9 peak(quercetin), No.11 peak(resveratrol), No.12 peak(quercetin-3-rhamnoside), No.13 peak(emodin-8-O-β-D-glucoside), No.16 peak(emodin), No.18 peak(emodin methyl ether). Conclusion The effect of P. cuspidatum on gouty arthritis is the result of many components. This study can provide reference for the quality control and application development of this medicine.