不同产地黄芩主要化学成分含量及抗肿瘤活性比较研究

The Contents and Anti-tumor Activity of Main Chemical Components of Scutellaria baicalensis from Different Origins:A Comparative Study

本研究旨在探究不同产地对黄芩的化学成分含量及抗肿瘤活性的影响。通过HPLC色谱法测定黄芩中黄芩苷、汉黄芩苷、黄芩素、汉黄芩素的含量差异,采用CCK-8法检测不同产地的黄芩对抑制A549肺癌细胞增殖的情况。结果表明,不同产地黄芩样品中的化学成分含量有显著差异,黄芩苷含量由高到低为黑龙江勃利>山东莒县>甘肃定西>山西运城,汉黄芩苷含量由高到低为黑龙江勃利>甘肃定西>山东莒县>山西运城,黄芩素含量由高到低为黑龙江勃利>山东莒县>山西运城>甘肃定西,汉黄芩素含量由高到低为黑龙江勃利>山西运城>山东莒县>甘肃定西。综上,黑龙江勃利地区黄芩中的化学成分含量高居于4个产地首位。黄芩提取物对A549肺癌细胞有抑制作用,且抑制作用与其化学成分含量有关,并呈现剂量依赖。实验结果可为寒地黄芩的开发及合理利用药用资源提供一些参考。

The purpose of the study is to investigate the effect of different origins on the contents of the chemical components and anti-tumor activity Scutellaria baicalensis.The study used HPLC chromatography to determine the contents of baicalin,wogonoside,baicalein and wogonin of S.baicalensis from different origins,and CCK-8 method to detect the inhibition of A549 lung cancer cell proliferation.The results show that there are significant differences in the contents of chemical components of S.baicalensis samples from different origins.The content of baicalin from high to low is Boli of Heilongjiang>Juxian of Shandong>Dingxi of Gansu>Yuncheng of Shanxi;the content of wogonoside from high to low is Boli of Heilongjiang>Dingxi of Gansu>Juxian of Shandong>Yuncheng of Shanxi;the content of baicalein from high to low is Boli of Heilongjiang>Juxian of Shandong>Yuncheng of Shanxi>Dingxi of Gansu;and the wogonin content from high to low is Boli of Heilongjiang>Yuncheng of Shanxi>Juxian of Shandong>Dingxi of Gansu.As shown above,the contents of the chemical components of S.baicalensis in Boli of Heilongjiang rank the first among those from the 4 origins.The extract of S.baicalensis has an inhibitory effect on A549 lung cancer cells,and the effect is related to the contents of the chemical components and dose-dependent.The experimental results can provide reference for the development and rational use of medicinal resources of S.baicalensis.