大豆GmNF-YB2基因生物信息学和表达分析及与GmNF-YA10互作鉴定

Bioinformatics and Expression Analysis of Soybean GmNF-YB2 Gene and Interaction Identification of GmNF-YA10

NF-Y三聚体复合物是由NF-YA、NF-YB和NF-YC 3个不同亚基组成,为探究大豆GmNF-YB2亚基在NF-Y三聚体复合物中的作用及其对干旱胁迫的响应,本研究克隆GmNF-YB2基因,对其进行生物信息学分析和表达模式分析,并鉴定GmNF-YA10与GmNF-YB2蛋白之间的互作关系。结果表明:GmNF-YB2基因序列全长1109 bp,编码区长498 bp,编码165个氨基酸。预测该蛋白分子量为18.19 kD,理论等电点6.10,进化树分析发现其与AtNF-YB2和AtNF-YB3处于同一分支。转录组数据和组织表达模式分析发现GmNF-YB2基因在检测的所有组织中均有表达,其中在子叶和叶片中的表达量较高;干旱胁迫时叶片中的表达量降低。酵母双杂交分析表明GmNF-YA10和GmNF-YB2之间不存在蛋白互作关系。

The NF-Y trimer complex is composed of 3 different subunits,NF-YA,NF-YB and NF-YC.In order to explore the interaction between soybean GmNF-YB2 subunits in the NF-Y trimer complex and the response to drought stress,in this study,we cloned GmNF-YB2 gene,performed bioinformatics analysis and expression pattern analysis,and identified the protein interaction relationship between GmNF-YA10 and GmNF-YB2.The results showed that GmNF-YB2 gene was 1109 bp in length,with a coding region of 498 bp,and encoding 165 amino acids.The molecular weight of the protein was predicted to be 18.19 kD and the theoretical isoelectric point was 6.10.The phylogenetic tree analysis showed that the protein was in the same branch with AtNF-YB2 and AtNF-YB3.Transcriptomic data and tissue expression pattern analysis showed that GmNF-YB2 gene was expressed in all the detected tissues,and the expression level was higher in cotyledons and leaves.The expression of GmNF-YB2 gene was decreased in leaves under drought stress.Yeast two-hybrid analysis showed that there was no protein interaction between GmNF-YA10 and GmNF-YB2.