接种内生菌对干旱胁迫下甘蔗的生理影响

Physiological Effects of Inoculated Endophytes on Sugarcane Under Drought Stress

探索内生菌对甘蔗抗旱能力的影响,以期为开发利用甘蔗内生菌抗旱性功能菌株提供理论依据和技术支撑。以课题组前期分离鉴定的6株甘蔗内生菌为供试菌株,甘蔗品种‘ROC22’幼苗接种7天后进行干旱胁迫,然后取完全展开的第一叶叶片测定相关生理指标。结果表明,E3、O9和YC89均有较高的ACC脱氨酶活性,不同甘蔗内生菌菌株对甘蔗抗旱性的影响有明显差异。E3、O9和YC89在提高甘蔗耐旱能力方面效果显著,主要表现在降低细胞膜损伤、增加渗透调节物质的含量、降低膜脂过氧化程度和增加SOD、POD活性等方面。试验发现,产ACC脱氨酶的甘蔗内生菌能更有效提高甘蔗抗旱能力。

The purpose of this study is to explore the influence of sugarcane endophytes on its drought resistance,and to provide a theoretical basis and technical support for the development and utilization of sugarcane endophytes with drought-resistant functional strains.Six sugarcane endophytes isolated and identified by the research group were used as test strains.Drought stress was simulated for seedlings of sugarcane variety‘ROC22’7 days after the inoculation,and then fully expanded first leaf was taken to determine the relevant physiological indicators.The results show that E3,O9 and YC89 all have high ACC deaminase activity,and the effects of different sugarcane endophytic strains on sugarcane drought resistance are significantly different.E3,O9 and YC89 have significant effects on improving the drought tolerance of sugarcane,mainly in terms of reducing cell membrane damage,increasing the content of osmotic adjustment substances,reducing the degree of membrane peroxidation,and increasing the activity of SOD and POD.This experiment found that the sugarcane endophytes producing ACC deaminase can effectively improve the drought resistance of sugarcane.