中华绒螯蟹微孢子虫全基因组分泌蛋白的预测分析

Genome-Wide Prediction and Analysis of Secretory Proteins of Microsporidium Hepatospora eriocheir Parasited in Chinese Mitten Crab Eriocheir sinensis

本试验运行真核生物分泌蛋白预测流程EuSecPred 2.0在中华绒螯蟹微孢子虫全基因组范围内预测分泌蛋白,同时分析分泌蛋白的序列特征,注释功能。结果表明,分泌蛋白序列长度大多为30~300 aa;信号肽长度为11~23 aa;信号肽切割位点处主要由疏水性氨基酸组成;对预测所得蛋白质功能的注释,发现了多种关键蛋白,如血凝素酯酶、甲基转移酶EZH1和丝氨酸/苏氨酸蛋白激酶等。基序分析发现,信号肽中存在基序S[NS]N[TI][FI]VI[IGD][RG]VRC。预测获得的这些重要分泌蛋白与中华绒螯蟹微孢子虫侵染宿主、调控宿主细胞周期及免疫反应等生理活动相关,可为筛选致病相关候选因子和探究其侵染机理提供重要依据。

In this study, we predict the secretory proteins of microsporidium Hepatospora eriocheir genome parasited in Chinese mitten crab Eriocheir sinensis with the EuSecPred 2.0 and annotate for secreted protein function, characteristics and the sequence analysis. The length of secreted proteins was found to be primarily changed from 30 amino acids to 300 amino acids, with length of signal peptide from 11 amino acids to 23 amino acids. The signal peptide cleavage site was mainly composed of hydrophobic amino acids. Many key proteins were found by functional annotation of the secretory proteins such as haemaglutinin-esterase, methyltransferase EZH1, and serine/threonine protein kinase. Motif analysis revealed that there was a S[NS]N[TI][FI]VI[IGD][RG]VRC motif in signal peptides which predicted secreted proteins were involved in the physiological activities of H. eriocheir, host cell cycle regulation and immune response. The findings provide important basis for screening disease-related candidate factors and exploring the infection mechanism.