高效液相色谱法测定复方黄黛片中丹酚酸B的含量

Determination of salvianolic acid B in compound realgar and natural indigo tablets by HPLC

目的建立高效液相色谱法(HPLC)用于测定复方黄黛片中丹酚酸B的含量。方法色谱柱为C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸(22∶78),流速为1.0 mL/min,检测波长为286 nm,柱温为35℃,进样量为10μL。结果丹酚酸B在10.076~151.140 mg/L范围内与峰面积呈良好线性关系(r=0.9999),平均加样回收率为95.62%,相对标准差为1.6%。结论该法简便、准确,可用于制剂的质量控制。

Objective To establish a high-performance liquid chromatography(HPLC)method for the determination of salvianolic acid B in the compound realgar natural indigo tablet.Methods A C_(18) column(4.6 mm×250 mm,5μm)was used,the mobile phase was acetonitrile-0.1%phosphoric acid(22∶78),the flow rate was 1.0 mL/min,the detection wavelength was 286 nm,the column tem⁃perature was 35℃,and the injection volume was 10μL.Results Salvianolic acid B showed a good linear relationship with the peak area in the concentration range of 10.076~151.140 mg/L(r=0.9999),the average recovery was 95.62%,and the relative standard devia⁃tion(RSD)was 1.6%.Conclusion The method is simple and accurate,and can be used for the quality control of compound realgar and natural indigo tablets.