基于乙酰胆碱酯酶抑制的有机磷农药生物传感器研究进展

A Review of Organophosphorus Pesticide Biosensors Based on Acetylcholinesterase Inhibition

有机磷农药(OPs)残留可通过食物链在生物体内富集,从而对自然生态和人类健康带来极大威胁。用于检测有机磷农药的传统分析方法(如高效液相色谱法和气相色谱法)具有响应时间较长、成本较高以及检测程序复杂等局限。近年来,生物传感因具有专一性强、速度快、准确度高等优势被广泛用于分析领域。而利用乙酰胆碱酯酶(AChE)作为识别元件和催化元件的生物传感器为检测实际样品中有机磷农药残留开创了新的前景。该综述概述了基于不同反应机理的乙酰胆碱酯酶抑制的各种最新传感方法和装置,特别是近年来结合新生代检测技术的传感装置,并总结了当前基于AChE抑制的方法在有机磷农药检测方面所面临的挑战和不足,展望了未来的研究方向。

Organophosphorus pesticides(OPs)residues have posed a great threat to natural ecosystem and human health by enrichment through the food chain.Traditional analytical methods for OPs detection,including high performance liquid chromatography(HPLC)and gas chromatography(GC)have some inevitable limitations, such as long response times, high cost, and requirements for complicated detection procedure and highly qualified operators.In recent years, biosensors have been effectively used in the field of quantitative analysis chemistry due to their strong specificity,high speed and high accuracy.The biosensors using acetylcholinesterase(AChE)as an identification element and catalytic element have opened up a bright prospect for the rapid and practical determination of OPs residues in real samples.In this review,the latest sensing strategies and devices based on AChE inhibition with different reaction mechanisms are summarized,especially the sensing devices combined with new generation detection technologies.The challenges and deficiencies for the current detection of OPs based on AChE inhibition are also summarized,and the future research direction is prospected.