圈养喜马拉雅塔尔羊粪便菌群结构及大肠杆菌耐药性试验

Fecal Microflora Composition and Drug Resistance of Escherichia coli in Captive Himalayan Tahr

为了解圈养塔尔羊肠道菌群结构以及种群耐药性流行情况,本试验以济南动物园内塔尔羊种群作为研究对象,分别采集成年组与亚成体组新鲜粪便各6份,利用高通量测序技术对两组动物粪便菌群结构进行对比分析。结果显示:塔尔羊粪便菌群在门水平上厚壁菌(Firmicutes)和拟杆菌(Bacteroidetes)为两大优势菌门,成年组和亚成体组厚壁菌的平均含量分别为(60.35±1.71)%和(59.96±2.48)%,拟杆菌的平均含量分别为(29.95±2.80)%和(29.60±2.51)%,两组间差异不显著(P>0.05)。在属水平上,孢杆菌属(Sporobacter)含量成年组显著高于亚成体组(P<0.05)。从20份不同个体粪便样本中各分离出1株大肠杆菌,共计20株,采用微量肉汤稀释法检测其对7大类14种抗菌药物的敏感性。结果显示:分离株对磺胺类和四环素类药物的耐药率均为5%,对β-内酰胺类、氨基糖苷类、喹诺酮类、多黏菌素类和酰胺醇类药物敏感,总耐药率为10%。本试验结果可为塔尔羊疾病的诊断和治疗用药提供参考。

This study aimed to investigate the intestinal flora composition and the prevalence of drug resistance in captive tahr.Six fresh fecal samples each were collected from adult and sub adult groups of the tahr population in Jinan zoo,and their flora composition was analyzed by high-throughput sequencing.The results showed that Firmicutes and Bacteroidetes were the two dominant phyla in tahr feces,making up(60.35±1.71)%and(59.96±2.48)%,respectively,and the average contents of Bacteroidetes were(29.95±2.80)%and(29.60±2.51)%,respectively,with no significant difference between the two groups(P>0.05).At the genus level,the content of Sporobacter in adult group was significantly higher than that in subadult group(P<0.05).Additionally,one strain of Escherichia coli was isolated from 20 fecal samples of different individuals,and its sensitivity to 14 kinds of antibiotics in 7 categories was assessed by broth microdilution method.The results showed that 10%of the isolates were drug resistant,including sulfonamides and tetracyclines at 5%,but sensitive toβ-lactams,aminoglycosides,quinolones,polymyxin and amido alcohols.This study provides valuable reference for the diagnosis and treatment of E.coli infection in captive tahr.