摘要
为了明确不同中药对标准菌及广东地区临床分离大肠杆菌(Escherichia coli)的抗菌活性及菌种优势血清型的地区差异性,本试验用管碟法比较14味中药对2株大肠杆菌标准菌株及22株广东地区分离的临床大肠杆菌的抑菌作用;用K-B纸片扩散法进行抗菌药药敏试验。结果显示,14种中药中仅白头翁(Anemone)、金银花(Lonicera japonica)、五倍子(Galla chinensis)、秦皮(Ash bar)、黄连(Coptis root)、大青叶(Isatis indigotica)6种对临床大肠杆菌菌株有抑菌作用,其中金银花和五倍子抑菌活性最强,穿心莲(Andrographs paniculata)、大黄(Rheum palmatum)、苦参(Lightyellow sophora root)等无抑菌作用;在抗菌药药敏试验中,临床大肠杆菌菌株对多黏菌素B(Polymyxin B)最为敏感。本试验为有效利用中药防治广东地区大肠杆菌病打下试验基础,对消除细菌耐药性提供科学依据。
This study examined the antibacterial activity of 14 traditional Chinese medicines against Escherichia coli reference strains and clinical isolates from Guangdong,including regional dominant serotypes.The antimicrobial activity of 14 traditional Chinese medicines was tested against 2 E.coli reference strains and 22 E.coli clinical strains from Guangdong province by cup-plate method,and antibiotic susceptibility of E.coli strains was evaluated by K-B disk diffusion method.The results showed that among 14 traditional Chinese medicines,only 6(Anemone,Lonicera japonica,Galla chinensis,Ash bar、Coptis root,and Isatis indigotica)demonstrated antibacterial effects on clinical E.coli strains.Among them,Lonicera japonica and Galla chinensis displayed the strongest antibacterial activity,while Andrographis paniculata,Rheum palmatum and Lightyellow sophora root had no antibacterial effect.In antibiotic susceptibility test,E.coli clinical strains were most sensitive to Polymyxin B.This study lays a foundation for the clinical application of traditional Chinese medicines against E.coli infection in Guangdong province,and opens a way for further investigation on the mechanism of antibiotic resistance in E.coli.
作者
罗怡琳
田浪
许娃丽
罗卓琳
吴保庆
LUO Yi-lin;TIAN Lang;XU Wa-li;LUO Zhuo-lin;WU Bao-qing(Guangdong Haida Group Co.,Ltd.,Guangzhou 511400,China)
出处
《中国兽医杂志》
CAS
北大核心
2021年第12期91-96,共6页
Chinese Journal of Veterinary Medicine
基金
广东省重点领域“精准农业”研发计划(2019B020218003)。
关键词
中药
大肠杆菌
体外抑菌效果
管碟法
药敏试验
traditional Chinese medicine
Escherichia coli
in vitro antibacterial effect
cup-plate method
antibiotic susceptibility test