叶黄素抑制ABHD11-AS1对胶质瘤细胞增殖、凋亡和侵袭的影响

Effects of Lutein on Proliferation, Apoptosis and Invasion of Glioma Cells by Inhibiting ABHD11-AS1

目的:探讨叶黄素对胶质瘤细胞增殖、侵袭和凋亡的影响及可能机制。方法:采用低、中、高剂量的叶黄素干预胶质瘤细胞48 h,细胞计数试剂盒和Transwell法检测细胞活力和侵袭,流式细胞术检测周期分布和凋亡,实时荧光定量PCR (RT-qPCR)检测含α/β水解酶结构域蛋白11反义RNA1 (ABHD11-AS1)表达。将ABHD11-AS1小干扰RNA转染胶质瘤细胞U251,检测干扰ABHD11-AS1表达对U251细胞增殖、凋亡和侵袭的影响。结果:低剂量叶黄素对U251细胞存活、周期分布、侵袭、凋亡、ABHD11-AS1表达无显著影响。中、高浓度的叶黄素处理后,U251细胞存活率、侵袭数、S期细胞比例、ABHD11-AS1表达显著降低,凋亡率和G0-G1细胞比例显著升高(P<0.05)。干扰ABHD11-AS1表达后U251细胞存活率、侵袭数、S期细胞比例显著降低,凋亡率和G0-G1细胞比例显著升高(P<0.05)。过表达ABHD11-AS1后,叶黄素处理的U251细胞存活率、侵袭数、S期细胞比例显著升高,凋亡率和G0-G1细胞比例显著降低(P<0.05)。结论:一定剂量的叶黄素可降低胶质瘤细胞的增殖和侵袭能力,诱导细胞凋亡,其机制与抑制ABHD11-AS1表达有关。

Objective:To investigate the effects of lutein on proliferation,invasion and apoptosis of glioma cells and its possible mechanism. Methods:Low,medium and high doses of lutein were used to intervene glioma cells for 48 hours. Cell counting kit and Transwell method were used to detect cell viability and invasion,flow cytometry was used to detect cycle distribution and apoptosis,and real-time fluorescence quantitative PCR(RT-qPCR) was used to detect the presence of luteinα/β Hydrolase domain protein 11 antisense RNA1(ABHD11-AS1). The effects of interfering with the expression of ABHD11-AS1 on the proliferation,apoptosis and invasion of U251 cells were detected. Results:Low dose lutein had no significant effect on the survival,cycle distribution,invasion,apoptosis and ABHD11-AS1 expression of U251 cells. After treatment with medium and high concentrations of lutein,the survival rate,invasion number,S-phase cell proportion and ABHD11-AS1 expression of U251 cells decreased significantly,and the apoptosis rate and G0-G1 cell proportion increased significantly(P<0.05). After interfering with the expression of ABHD11-AS1, the survival rate, invasion number and S-phase cell proportion of U251 cells decreased significantly,and the apoptosis rate and G0-G1 cell proportion increased significantly(P<0.05). After overexpression of ABHD11-AS1,the survival rate,invasion number and S-phase cell proportion of U251 cells treated with lutein increased significantly,and the apoptosis rate and G0-G1 cell proportion decreased significantly(P<0.05).Conclusion: A certain dose of lutein can reduce the proliferation and invasion of glioma cells and induce apoptosis. Its mechanism is related to the inhibition of ABHD11-AS1 expression.