结直肠癌组织中长链非编码RNA NEAT1和miR-218-5p的表达及其与患者预后的关系

Relationship between expression of lncRNA NEAT1 and miR-218-5p in colorectal cancer and prognosis of patients

目的探究结直肠癌组织中长链非编码RNA(lncRNA)核富集转录本1(NEAT1)和miR-218-5p的表达水平与患者临床病理特征及预后的关系。方法选取2014年7月至2015年7月在中国人民解放军联勤保障部队第九〇〇医院确诊并接受治疗的95例结直肠癌患者的肿瘤组织及癌旁正常组织。采用实时荧光定量PCR(real time qPCR)法检测组织中lncRNA NEAT1和miR-218-5p的表达水平,并分析两者与患者临床病理特征及预后的关系。采用Cox比例风险回归模型分析影响结直肠癌患者预后生存的危险因素。结果与癌旁组织比较,结直肠癌组织中lncRNA NEAT1的表达水平较高,miR-218-5p的表达水平较低,两组差异均有统计学意义(P均<0.05)。结直肠癌组织中lncRNA NEAT1和miR-218-5p的表达水平与肿瘤分化程度、TNM分期、浸润深度、淋巴结转移密切相关(P<0.05)。Kaplan-Meier生存分析结果显示,高表达lncRNA NEAT1组患者的5年病死率显著高于低表达lncRNA NEAT1组(63.64%比41.18%,P<0.05),低表达miR-218-5p组患者的5年病死率显著高于高表达miR-218-5p组(65.31%比36.96%,P<0.05)。Cox比例风险回归模型分析结果显示,肿瘤分化程度、TNM分期、浸润深度、淋巴结转移、高表达lncRNA NEAT1、低表达miR-218-5p均为结直肠癌患者预后生存的危险因素。Pearson相关性分析结果显示,lncRNA NEAT1与miR-218-5p呈负相关(r=-0.563,P=0.000)。结论结直肠癌组织中lncRNA NEAT1呈高表达,miR-218-5p呈低表达,且两者均与患者的临床病理特征及预后生存有关。

Objective This paper is to investigate the expression levels of lncRNA NEAT1 and miR-218-5p in colorectal cancer and their relationship with clinicopathological features and prognosis.Methods The tumor tissues and adjacent normal tissues of 95 patients with colorectal cancer diagnosed and treated in No.900 Hospital,Joint Service Support Force,Chinese People's Liberation Army from July 2014 to July 2015 were selected.The expression levels of lncRNA NEAT1 and miR-218-5p in tissues were detected by real time qPCR,and their relationship with clinicopathological features and prognosis were analyzed.The Cox proportional hazards regression model was used to analyze the risk factors affecting the prognosis and survival of patients with colorectal cancer.Results Compared with adjacent tissues,the expression level of lncRNA NEAT1 is higher and that of miR-218-5p is lower in colorectal cancer tissues,with a statistically significant difference(P<0.05).The expression levels of lncRNA NEAT1 and miR-218-5p in colorectal cancer tissues are closely related to tumor differentiation,TNM stage,depth of invasion,and lymph node metastasis(P<0.05).The Kaplan-Meier analysis shows that the 5-year mortality rate of patients in the high expression lncRNA NEAT1 group is significantly higher than that in the low expression lncRNA NEAT1 group(63.64%versus 41.18%,P<0.05),and that of patients in the low expression miR-218-5p group is significantly higher than that in the high expression miR-218-5p group(65.31%versus 36.96%,P<0.05).The Cox proportional hazards regression analysis indicates that the degree of tumor differentiation,TNM stage,depth of invasion,lymph node metastasis,high expression of lncRNA NEAT1,and low expression of miR-218-5p are all risk factors for the prognosis and survival of colorectal cancer patients.The Pearson correlation analysis suggests that lncRNA NEAT1 and miR-218-5p are negatively correlated(r=-0.563,P=0.000).Conclusion lncRNA NEAT1 is highly expressed and miR-218-5p is low expressed in colorectal cancer,both of which are related to the clinicopathological features and prognosis of patients.