桂附地黄丸对环磷酰胺致免疫抑制小鼠血常规和血清生化指标影响

EFFECTS OF GUIFU DIHUANG PILLS ON BLOOD ROUTINE AND SERUM BIOCHEMICAL INDICATORS IN CYCLOPHOSHAMIDE INDUCED IMMUNOSUPPRESSIVE MICE

探究桂附地黄丸对环磷酰胺致免疫抑制小鼠的血常规及血清生化指标的影响。将24只雌性的昆明种小白鼠分为对照组(CT)、模型组(CTX)和给药组(GDT)。按照1.82 g/kg·d体重剂量给GDT小鼠灌胃桂附地黄丸悬液;在灌胃的第12 d,将环磷酰胺按照50 mg/kg·d体重剂量给CTX和GDT小鼠进行腹腔注射,连续注射3 d。最后一次用药12 h后,称重后采集血液检测血常规及生化指标。结果显示,与CT相比,CTX小鼠白细胞总数、单核细胞总数、淋巴细胞总数、血小板分布宽度、总蛋白含量显著降低(P<0.05),尿素氮含量显著升高(P<0.05);与CTX相比,GDT小鼠单核细胞总数、中性粒细胞总数、中性粒细胞百分比、平均红细胞血红蛋白含量、平均红细胞血红蛋白浓度、肌酐含量显著提升(P<0.05),淋巴细胞百分比、谷草转氨酶含量显著下降(P<0.05)。因此,桂附地黄丸可以有效改善环磷酰胺引起小鼠血常规和血清生化指标的异常变化。

To discovery the effects of Guifu Dihuang Pills on the blood routine and serum biochemical indicators in cyclophosphamide-induced immunosuppressive mice.Twenty-four female Kunming breed mice were divided into a control group(CT),a model group(CTX)and a Guifu Dihuang Pills treated group(GDT).The GDT mice were given Guifu Dihuang pill suspension by gavage according to the dose of 1.82 g/kg body weight(once a day for 14 days).On day 12 of intragastric,the CTX and the GDT were given intraperitoneal injection with 50 mg/kg b.w.cyclophosphamide(once a day for 3 days).12 hours after the last dose,after weighing,blood was collected to detect blood routine examination and biochemical indexes.The results showed that compared with CT,the white blood cell count,monocyte count,lymphocyte count,platelet distribution width and total protein contents were significantly lower(P<0.05)and the contents of urea nitrogen were significantly higher(P<0.05)in CTX mice.Compared with CTX,the total monocytes,total neutrophils,percentages of neutrophils,mean corpuscular hemoglobin contents,mean corpuscular hemoglobin concentrations and the creatinine contents were significantly higher(P<0.05),and the lymphocyte percentages,aspartate aminotransferase contents were significantly lower(P<0.05)in GDT mice than those in CTX mice.Thus,Guifu Dihuang Pills could effectively improve the abnormal changes of blood routine and serum biochemical indexes in cyclophosphamide-induced mice.