玉米bZIP基因应答逆境胁迫的表达模式分析

The Expression Pattern of ZmbZIP Gene under Adversity Stresses

为了探讨玉米bZIP基因家族成员在玉米抗逆中的作用,以玉米骨干自交系郑58为试验材料,设置200 mmol/L的NaCl溶液、20%PEG6000(Polyethylene Glycol 6000)、4℃低温和硝态氮或铵态氮缺乏胁迫试验,利用qPCR技术,对9个ZmbZIP基因应答各类逆境胁迫的响应表达模式进行了分析。系统进化树分析结果表明,9个ZmbZIP基因进一步细分为3个亚组。qPCR分析结果表明,8个基因在不同组织器官中表达,ZmbZIP80没有被检测到,其可能是假基因。在人为模拟盐、干旱、低温和氮胁迫条件下,8个ZmbZIP在应答不同胁迫时,呈现不同的表达模式,ZmbZIP37和ZmbZIP53受NaCl胁迫明显诱导,而ZmbZIP49和ZmbZIP79则受到显著抑制;硝态氮缺乏胁迫显著抑制叶片中ZmbZIP37和ZmbZIP53基因表达,ZmbZIP42和ZmbZIP49则受到铵态氮缺乏胁迫的明显诱导。这些结果表明,8个ZmbZIP在玉米抵御逆境胁迫时发挥着广泛的作用;9个ZmbZIP基因在玉米不同组织中和响应不同逆境胁迫时的表达模式明显不同。研究结果可为进一步研究这些基因的生物学功能提供科学数据。

In order to study the role of ZmbZIPs in the stress tolerance of maize,using maize inbreed line Zheng 58 for exprerimental material,expression analysis of 9 ZmbZIPs were performed under 200 mmol/L NaCl,20%PEG6000,4℃low temperature and nitrate or ammonium deficiency stresses.Evolutionary tree analysis indicated that 9 ZmbZIPs were divided 3 subgroup.RT-qPCR analysis showed that 8 ZmbZIPs were detected in maize tissues,whereas ZmbZIP80 were not,indicated it could be pseudogene.Under simulated salt,drought,low temperature and nitrogen stress conditions,8 ZmbZIPs exhibited different expression pattern in response to various stresses,ZmbZIP37 and ZmbZIP53 were induced while ZmbZIP49 and ZmbZIP79 were downregulated obviously under NaCl stress,ZmbZIP37 and ZmbZIP53 of leaves significantly suppressed under nitrate deficit stress while ZmbZIP42 and ZmbZIP49 were upregulated in responsive to ammonium deficient stress.The result indicated 8 ZmbZIP played widely roles against stress conditions.Expression pattern of 9 ZmbZIPs gene differed in different tissues or under various adverse stresses.The study can offer scientific data for further reveal ZmbZIP biological function.